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Molecular method for identifying Mucomyzius japonicus in North China of China

A kind of hibiscus, auxiliary identification technology, applied in biochemical equipment and methods, microbial determination/inspection, DNA/RNA fragments, etc.

Pending Publication Date: 2022-04-12
河北省农林科学院经济作物研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] There are many species of the genus Helicoptera, which are widely distributed. About 40,000 species are known in the world, and the identifiable characteristics are not obvious. Many species have little morphological difference, and there are also morphological variations in the same species. Not easy to identify

Method used

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  • Molecular method for identifying Mucomyzius japonicus in North China of China
  • Molecular method for identifying Mucomyzius japonicus in North China of China
  • Molecular method for identifying Mucomyzius japonicus in North China of China

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1. Obtaining of the mitochondrial COI gene of A. japonica

[0046] 1. DNA extraction

[0047] Extraction and selection of well-preserved 2 samples of A. japonica from Luquan District, Shijiazhuang, North China, and 1 sample of . Perform single-head DNA extraction. The concentration and quality of genomic DNA were detected by NanoDrop Spectrophotometer (DS-11Envivx), the integrity of genomic DNA was detected by 1% agarose gel electrophoresis, and stored at -20°C for future use.

[0048] 2. Amplification of mitochondrial COI gene fragments

[0049] Using the genomic DNA in 1 above as a template, perform PCR amplification with the following general primers to obtain PCR amplification products.

[0050] The PCR amplification of the above-mentioned mitochondrial COI gene fragment adopts universal primers,

[0051] Upstream primer LCO1490: GGTCAACAAATCATAAAGATATTGG;

[0052] Downstream primer HCO2198: TAAACTTCAGGGTGACCAAAAATCA.

[0053] The PCR amplification sys...

Embodiment 2

[0069] Example 2, Application of Mitochondrial COI Gene in Identifying A. japonica

[0070] 1. Genomic DNA extraction

[0071] Extract 3 Pimpla nipponica, 3 Pimpla disparis, and 3 Pimpla disparis from Luquan District of Shijiazhuang and Fudong District of Handan City (2018 and 2019) Genomic DNA of a bee (Diplazonlatatorius).

[0072] 2. Amplification of mitochondrial COI gene

[0073] Using the above-mentioned genomic DNA as a template, the following general primers were used to amplify according to the method of Example 1:

[0074] Upstream primer LCO1490: GGTCAACAAATCATAAAGATATTGG;

[0075] Downstream primer HCO2198: TAAACTTCAGGGTGACCAAAAATCA.

[0076] Obtain the PCR amplification product.

[0077] PCR amplification products of different bees were sequenced.

[0078] The result is as follows:

[0079] The sequencing results of the three A. japonicus were sequence 1;

[0080] The sequencing results of the three gypsy moths were all sequence 3;

[0081] The sequencing...

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Abstract

The invention discloses a molecular method for identifying Japan myzigus marmoreus in North China of China. The invention provides a protein which is as shown in (1) or (2): (1) a protein consisting of an amino acid sequence as shown in a sequence 2 in a sequence table; and (2) a protein which is derived from (1), has the same function and is obtained by substitution and / or deletion and / or addition of one or more amino acid residues on the amino acid sequence as shown in the sequence 2 in the sequence table. Experiments prove that compared with a traditional ecological identification method, a molecular method for identifying the Japanese neomyzigus marmoreus in the North China region of China is rapid, convenient and accurate, and a large number of samples can be rapidly identified at a time; meanwhile, part of available samples cannot influence the recognition result; specific DNA sequence information is used, so that identification errors of species caused by morphological variation can be avoided; and non-ecological experts can also identify the species, and the accuracy is high.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a molecular method for identifying A. japonicus in North China. Background technique [0002] At present, the most effective and accurate identification method of biodiversity and species is traditional morphological taxonomy identification. However, traditional morphological taxonomy conducts species identification based on the morphological characteristics, life history and biological characteristics of organisms, which is time-consuming and laborious, and has high requirements for the integrity of specimen preservation and professional level, and may be affected by subjective factors, resulting in wrong identification (Schindel and Miller, 2005). DNA barcode is a specific DNA fragment sequence in the genome of a species, which has the characteristics of stable variation and easy amplification, and can effectively make up for the shortcomings of traditional taxonomy (Gere et al., 20...

Claims

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Application Information

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IPC IPC(8): C12N15/11C12Q1/6888
Inventor 高慧敏张颖君郝玲玉要荣慈王敏田玉曹岩坡张晗
Owner 河北省农林科学院经济作物研究所
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