Diaphorina citri trapping agent and application thereof

A citrus psyllid trapping technology, applied in the citrus psyllid attractant and its application field, can solve the problems of complex preparation and many kinds of ingredients, achieve good control effect, reduce population number, and reduce the use amount

Pending Publication Date: 2022-04-12
岭南现代农业科学与技术广东省实验室 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The Chinese patent "A Citrus Psyllid Attractant" discloses a citrus psyllid attractant based on the volatile substances released by citrus itself, but the attractant contains many types of ingredients and is relatively complicated to prepare. There are reports on the use of citrus psyllid pheromones in the control of citrus psyllids

Method used

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  • Diaphorina citri trapping agent and application thereof
  • Diaphorina citri trapping agent and application thereof
  • Diaphorina citri trapping agent and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Example 1 Extraction and test analysis of citrus psyllid semiochemicals

[0055] 1. Extraction of citrus psyllid sex semiochemicals

[0056] After the female and male citrus psyllids were separated by an insect stereoscope, the extracts of the female and male adults of the citrus psyllid were respectively extracted by a solvent extraction method, and the extracts contained sex semiochemicals of the citrus psyllid.

[0057] Put 50 citrus psyllids of the same sex into a 10mL centrifuge tube, inject 500μL of n-hexane (chromatographic grade) and soak for 30min, shake the centrifuge tube constantly by hand during this period, and then transfer the supernatant to a 2mL centrifuge tube with a pipette gun , and concentrated to 50 μL with nitrogen gas to obtain the extract of citrus psyllid adults. The extracts were airtight and stored in a refrigerator at low temperature (-20°C) for subsequent behavioral measurements and field trials.

[0058] 2. Determination of the selectio...

Embodiment 2

[0071] Example 2 The effect of yellow board and quasi-semiochemicals on the trapping effect of citrus psyllids

[0072] 1. The effect of yellow board and the extracts of female and male insects on the total amount of citrus psyllid trapping

[0073] The test was carried out in the net room, and the net room was separated into two areas (divided into A treatment area and B treatment area) with gauze. Before the test started, the population density of citrus psyllids in the two treatment areas of net room A and B were observed and adjusted to ensure that the population density of citrus psyllids among the plants in the area was approximately the same.

[0074] Preparation of lures and blank lures: extracts of female and male adults of citrus psyllid were extracted by the method described in Example 1 as attractants. When making the lure, first rinse the rubber head with the organic solvent n-hexane for 24 to 36 hours, and then inject the attractant into the rubber head to make ...

Embodiment 3

[0091] Example 3 The effect of yellow board suspension mode and adhesive type on the trapping effect of citrus psyllids

[0092] 1. The effect of different hanging heights of yellow boards on trapping citrus psyllids

[0093] Select the yellow plate that size is 20cm * 15cm, the yellow plate is suspended between the plants of A treatment area, and the hanging mode has three kinds: (1) the bottom of the yellow plate is higher than the top 30cm of the Murata plant (collectively referred to as the top); (2) ) the top of the yellow board is flush with the top of the Murica plant (collectively referred to as the upper part); (3) the top of the yellow board is flush with the middle part of the Murica plant (collectively referred to as the middle part). The distance between the yellow boards is 3m, and the row spacing is 1m. The yellow boards of each hanging height are repeated 3 times, and they are randomly arranged in a row. A total of 9 yellow boards are hung. Six days after hang...

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Abstract

The invention discloses a diaphorina citri trapping agent and application thereof. On the basis that the diaphorina citri female extract has a trapping effect on diaphorina citri, the diaphorina citri trapper and the diaphorina citri pest trapping method are developed, namely, the diaphorina citri physical and chemical trapping technology combining insect pheromone and a color trapping technology is provided, and the diaphorina citri physical and chemical trapping technology can be used for preventing and treating the diaphorina citri pests. According to the method, the diaphorina citri female extract or methyl palmitate is used as a trapping agent, green prevention and control are carried out on the diaphorina citri in combination with a yellow double-sided pest sticking plate, operation and popularization are easy, in the diaphorina citri population prevention and control process, the use amount of chemical pesticides can be remarkably reduced, the damage rate of rutaceous plants is reduced, and the economic benefits of the diaphorina citri are improved. The biodiversity and natural enemy resources are protected, the prevention and control efficiency of diaphorina citri is improved, and an important way is opened up for a comprehensive prevention and control technical system of diaphorina citri pests.

Description

technical field [0001] The invention belongs to the technical field of green prevention and control of agricultural pests. More specifically, it relates to a citrus psyllid attractant and its application. Background technique [0002] The citrus psyllid (Diaphorinacitri Kuwayama) is a piercing-sucking insect of the order Hemiptera, which mainly harms Rutaceae plants, among which Citrus suffers the most, followed by Pseudomonas, Murata and Lycium. The citrus psyllid directly feeds on the young shoots of the host plant, and the young shoots of the damaged plants may wither, and the new shoots are distorted; during the development of the citrus psyllid, it will also secrete white honeydew and adhere to the branches and leaves, resulting in soot on the plant sick. What's more serious is that the citrus psyllid will carry the huanglongbing bacteria for life after eating the diseased plants infected with citrus huanglongbing, and the transmission rate is very high. The citrus p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/14A01N37/02A01N27/00A01N29/02A01P19/00A01M1/14A01M1/02A01G13/00
Inventor 邱宝利欧达许炜明王磊桑文
Owner 岭南现代农业科学与技术广东省实验室
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