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Construction method of visual electrochemical luminescence sensor based on ruthenium (II) coordination compound and application of visual electrochemical luminescence sensor in detection of OTA

A construction method and aptamer sensor technology, applied in the application field of detection of ochratoxin A, can solve the problems of inability to realize rapid on-site detection, single detection method, complicated operation, etc., achieve flexible use mode, reduce complexity, The effect of high sensitivity

Pending Publication Date: 2022-02-25
JIANGSU UNIV
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Problems solved by technology

The detection method of the above detection method is single, the operation is complicated, it is not portable, and it cannot realize on-site rapid detection

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  • Construction method of visual electrochemical luminescence sensor based on ruthenium (II) coordination compound and application of visual electrochemical luminescence sensor in detection of OTA
  • Construction method of visual electrochemical luminescence sensor based on ruthenium (II) coordination compound and application of visual electrochemical luminescence sensor in detection of OTA
  • Construction method of visual electrochemical luminescence sensor based on ruthenium (II) coordination compound and application of visual electrochemical luminescence sensor in detection of OTA

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Embodiment Construction

[0041] The present invention will be described in detail below in conjunction with the accompanying drawings and examples, but the present invention is not limited to these examples.

[0042] figure 1 The mechanism diagram for the detection of the constructed visualized electrochemiluminescent aptasensor, figure 2 It is a schematic diagram of the ITO electrode region of the present invention.

[0043] (1)Ru(bpy) 3 2+ / Preparation of BiOI microspheres

[0044] 1.455g of bismuth nitrate pentahydrate and 0.492g of potassium iodide were respectively dissolved in a beaker containing 20mL of ethylene glycol solution, and stirred to form a homogeneous solution. Then, the bismuth nitrate solution was added dropwise to the potassium iodide solution, stirred vigorously, and the solution gradually changed from an initial clear solution to an orange suspension. The stirred suspension was transferred to a 50 mL polytetrafluoroethylene autoclave, and reacted at a temperature of 140 °...

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Abstract

The invention provides a construction method of a visual electrochemical luminescence sensor based on a ruthenium (II) coordination compound, wherein the method adopts the fixation and enhancement effect of BiOI on Ru(bpy)3<2+>, does not need to adopt a traditional photomultiplication (PMT) amplification strategy, designs two regions on ITO, and realizes separation of a visual unit and a detection unit. The invention discloses electrochemical luminescence visual detection based on luminous intensity change of a ruthenium (II) coordination compound illuminant. The electrochemical luminescence visual detection comprises the following steps: 1, preparing a luminescent material bipyridine ruthenium bismuth oxyiodide composite microsphere (Ru(bpy)3<2+> / BiOI); and 2, constructing a visual electrochemical luminescence aptamer sensing device for detecting ochratoxin A. According to the invention, on the basis, a portable detection mode integrating the advantages of rapidness, simplicity, miniaturization, flexible use mode and the like is provided, and on-site rapid detection can be realized.

Description

technical field [0001] The invention belongs to the technical field of electrochemiluminescence biosensing, and mainly relates to a construction method of a novel visual electrochemiluminescence sensor based on a ruthenium (II) complex and its application in detecting ochratoxin A. Background technique [0002] Electrochemiluminescence, or electrochemiluminescence (ECL), is a process in which the products of electrode reactions or between electrode products and a certain component in the system undergo a chemical reaction to produce light radiation, and is the product of the combination of chemiluminescence and electrochemistry. It not only retains the advantages of the chemiluminescence method, but also has many incomparable advantages of the chemiluminescence method. [0003] The electrochemiluminescence sensor is a kind of experimental device constructed by directly or indirectly immobilizing the reagents involved in the chemiluminescence reaction on the working electrode...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327G01N27/30G01N27/416G01N21/76
CPCG01N27/3275G01N27/305G01N27/416G01N21/76
Inventor 郝楠邱宇左艳丽邹艺王坤
Owner JIANGSU UNIV
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