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Plant salt-tolerant gene module mining method

A salt-tolerant gene and gene technology, applied in the fields of genomics, bioinformatics, instruments, etc., can solve the problems of limited effect and inability to systematically understand the molecular mechanism of plant drought resistance, and achieve the effect of improving efficiency and accuracy

Pending Publication Date: 2022-01-28
TROPICAL CORP STRAIN RESOURCE INST CHINESE ACAD OF TROPICAL AGRI SCI +1
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Problems solved by technology

For this reason, the functional analysis of a single or a few salt-tolerance-related genes cannot systematically understand the molecular mechanism of plant drought resistance, and the use of a single salt-tolerance-related gene to genetically engineer crops has also proved to be limited. Therefore, the present invention proposes a A method for discovering salt-tolerant gene modules in plant species

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Embodiment Construction

[0015] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0016] The invention discloses a method for excavating a plant salt-tolerant gene module, which comprises the following steps: the first step is to separate and establish the gene interaction database of the target plant; the second step is to use the Markov clustering algorithm to model the gene interaction network and evaluate the biological significance of the gene module; the third step is to use gene chip data to identify the salt stress response genes of ...

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Abstract

The invention discloses a plant salt-tolerant gene module mining method. The method comprises the following steps that: based on a gene interaction network, a Markov clustering algorithm is adopted to carry out modular analysis on the gene interaction network, and gene chip and whole genome associated data are utilized to evaluate the salt tolerances of gene modules; and a key salt-tolerant gene module is screened out according to the salt-tolerant contribution of each gene module. Compared with an existing method, the method focuses on discovery of a brand new salt-tolerant gene group, and greatly improve the efficiency and accuracy of plant salt-tolerant gene mining.

Description

technical field [0001] The invention relates to the technical field of salt-tolerant genes, in particular to a method for discovering plant salt-tolerant gene modules. Background technique [0002] The salinization of cultivated land is one of the main problems faced by my country's agricultural production at present, which seriously affects my country's agricultural production and food security. Cultivating new varieties of crops that are resistant to salinity is one of the effective means to face salinization. For saline-alkali land improvement is of great significance. The mechanism of plants adapting to saline-alkali environment is very complex, which is the result of the comprehensive regulation of complex networks formed by the interaction of many genes / pathways. After plants are subjected to salt stress, through signal perception and a series of complex signal transduction and activation of specific transcriptional regulatory factors, and then regulate the expression ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G16B20/20
CPCG16B20/20
Inventor 刘一明李晓霞严琳玲黄春琼郇恒福黄睿刘国道
Owner TROPICAL CORP STRAIN RESOURCE INST CHINESE ACAD OF TROPICAL AGRI SCI
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