Regulation and control method for promoting excessive absorption and accumulation of lead by Arabis alpina Linn. var. parviflora Franch. through mediation of sulfur
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A technology of Arabidopsis thaliana and sulfur, applied in the field of plants
Pending Publication Date: 2022-01-07
YUNNAN AGRICULTURAL UNIVERSITY
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However, there is still no regulatory method to promote the overabsorption and accumulation of lead in A. floret.
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Embodiment 1
[0037] The sulfur-mediated method for promoting the excessive absorption and accumulation of lead in plants A. floretus comprises the following steps:
[0038] S1, soil selection:
[0039] Take the farmland soil near the abandoned slagpile, which is the soil for planting corn in the 0-20cm plow layer, pass through a 2mm sieve after air drying, and put it in a plastic pot for later use;
[0041] Use the seeds of A. thaliana to grow seedlings in substrate soil until the seedling stage, select seedlings with similar growth conditions, transplant them into pots respectively, and water them with 1 / 2 Hoagland nutrient solution every 2 days;
[0064] This embodiment provides a test experiment on the regulation method of the above-mentioned sulfur-mediated promotion of excessive absorption and accumulation of lead in the plant A. floretii. The specific experimental steps are as follows:
[0065] 1 Materials and methods
[0066] 1.1 Experimental materials
[0067] Soil for test: taken from Sanduoduo Village, Zhehai Town, Huize County, Yunnan Province (altitude 2463-2516m, east longitude 103°03′-103°55′, north latitude 25°48′-28°38′, near the abandoned slagpile) The farmland soil is the soil for planting corn in the 0-20cm plow layer. After air-drying, pass through a 2mm sieve, accurately weigh 5.0kg, and put it in a plastic pot (length 44cm×width 15cm×height 19cm) for use. Its main physical and chemical properties are listed in Table 1.
[0068] Table 1 Physical and chemical properties of the tested soil
[0069]
[0070] Plants to be tested: Use the seeds of A. thaliana to grow seedlings in substrate soil to t...
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Abstract
The invention discloses a regulation and control method for promoting excessive absorption and accumulation of lead by Arabis alpina Linn. var. parviflora Franch. through mediation of sulfur. The regulation and control method comprises the following steps of: soil selection, plant planting, sulfur element solution preparation, sulfur element treatment, biomass determination, determination of sulfur and lead in subcells, determination of lead content, data statistics and analysis and the like. According to the method of the invention, the biomass of the overground part and the underground part of the Arabis alpina Linn. var. parviflora Franch. under the treatment of sulfur with different valence states and concentrations, theoretical and technical method guidance is provided for the enrichment characteristic of lead and the distribution of lead in subcellular components, the research of the coping mechanism of the Arabis alpina Linn. var. parviflora Franch. to lead stress under the action of sulfur, the action mechanism of lead enrichment of the Arabis alpina Linn. var. parviflora Franch. on the subcellular level and the promotion of the restoration of lead-polluted soil by the hyperaccumulatedArabis alpina Linn. var. parviflora Franch.through sulfur regulation and control.
Description
technical field [0001] The invention relates to the technical field of plants, in particular to a sulfur-mediated regulation method for promoting excessive absorption and accumulation of lead in plants A. floretii. Background technique [0002] Lead has a large relative molecular weight and stable properties, and its geochemical cycle in the pedosphere is largely affected by human factors. Analysis of lead pollution sources in the environment has also confirmed that industry and agriculture have a large contribution rate. Lead migrates from soil to plants and accumulates in organisms, eventually endangering human health through the food chain. There have been many reports on the factors that affect the migration and transformation of lead in soil, such as the form of lead, soil type, soil properties, soil nutrients, fertilization, etc. In recent years, it has been reported that sulfur application can change the growth of crops. Sulfur can also be used as a non-metallic elem...
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