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Molecular marker related to porcine intermuscular fat and application of molecular marker

An intermuscular fat and molecular marker technology, applied in the field of livestock molecular marker preparation, can solve the problems of difficult separation of intermuscular fat, difficulty in specific research and evaluation, etc., and achieve the effect of high specificity and accuracy

Pending Publication Date: 2022-01-07
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Unlike subcutaneous fat and visceral fat, intermuscular fat is anatomically difficult to separate, making it difficult to specifically study and evaluate it

Method used

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  • Molecular marker related to porcine intermuscular fat and application of molecular marker
  • Molecular marker related to porcine intermuscular fat and application of molecular marker
  • Molecular marker related to porcine intermuscular fat and application of molecular marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1 Based on 10×Genomics single-cell RNA sequencing technology, the specific expression gene SNCA of porcine intermuscular adipocytes was obtained

[0040] Due to the difficulty in anatomically separating the intermuscular adipose tissue of livestock and poultry, ordinary transcriptome sequencing technology cannot achieve specific analysis of porcine intermuscular adipose tissue. However, 10×Genomics single-cell RNA sequencing technology is based on the Gemcode microfluidic platform, which can Single intermuscular adipocytes and gel beads containing 10× barcode information are wrapped in oil droplets to form a "water-in-oil" structure (GEMs). In GEMs, the cells are cleaved to release mRNA, which is generated by reverse transcription for sequencing cDNA, through high-throughput sequencing to obtain the gene expression of a single cell.

[0041]1) The lean-meat Landrace pigs with large differences in carcass meat quality and the fat-type Lantang pigs of Guangdong sp...

Embodiment 2

[0047] Example 2 Fluorescence quantitative PCR technique verification SNCA gene is used as the molecular marker reagent of porcine intermuscular fat:

[0048] Reverse transcription kit: PrimeScript TM RT reagent Kit with gDNA Eraser (PerfectReal Time), #RR047Q, Takara;

[0049] Quantitative PCR reagents: Fast qPCR Mix, #RR430A, Takara.

[0050] Relevant experimental items to RNase treatment:

[0051] 1) Rinse and soak all glassware with DEPC before use, high pressure at 120°C for 20 minutes, and dry at high temperature at 180°C for more than 2 hours.

[0052] 2) Soak plastic utensils (such as EP tubes / tips) in 0.1% DEPC water overnight before use, and then dry the liquid, 120°C and high pressure for 20 minutes, and dry in an oven for later use.

[0053] Total RNA extraction:

[0054] Select 50 lean-meat Landrace pig muscle tissues and 50 fat-type Blue Tang pig muscle tissues, and use the TRIzol method according to the instructions of the Invitrogen kit. The specific ste...

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Abstract

The invention discloses a molecular marker related to porcine intermuscular fat and application of the molecular marker, and belongs to the technical field of livestock molecular marker preparation. The back muscular tissue of a variety of pigs is detected by using a 10*Genomics single-cell RNA sequencing technology, and an intermuscular fat cell specific expression gene SNCA is obtained by screening; the SNCA gene or an encoding protein thereof can be used as the molecular marker of the porcine intermuscular fat, and is used for identifying the porcine intermuscular fat tissue and evaluating the porcine intermuscular fat content; and the molecular marker has higher specificity and accuracy, has important application value in molecular marker assisted breeding related to regulation and control of the porcine intermuscular fat content, and has important significance for breeding animal varieties with excellent meat quality characters and improving the meat quality.

Description

technical field [0001] The invention belongs to the technical field of livestock molecular marker preparation, and in particular relates to a molecular marker related to porcine intermuscular fat and an application thereof. Background technique [0002] Pork is the main source of meat in the daily diet of Chinese residents, and the improvement of pork quality traits is the most important research direction in livestock breeding. At present, domestic commercial pigs are mainly lean-meat pig breeds imported from abroad and their hybrid offspring. The imported breeds have the advantages of fast growth, carcass lean meat rate and high feed conversion rate; but compared with local pig breeds in my country, pigs are resistant to The stress ability and meat quality were significantly reduced. In recent years, with the increasing requirements of consumers for meat quality, it is urgent to improve meat quality and cultivate high-quality meat pigs. [0003] The intermuscular fat cont...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12Q1/6851G01N33/68
CPCC12Q1/6888C12Q1/6851G01N33/68C12Q2600/124C12Q2600/166C12Q2600/158G01N2333/47C12Q2531/113C12Q2521/107C12Q2563/107C12Q2545/101C12Q2527/107Y02P60/87
Inventor 孙加节林泽堃谢芳张永亮江青艳习欠云陈婷罗君谊
Owner SOUTH CHINA AGRI UNIV
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