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TE molecular marker closely linked with peach double petals and application thereof

A technology of molecular markers and double petals, which is applied in the direction of recombinant DNA technology, microbiological determination/inspection, DNA/RNA fragments, etc., can solve the problems such as the difficult molecular identification of peach double petals, achieve good application and promotion prospects, and have few restrictions , the effect of the simple method

Pending Publication Date: 2021-12-31
SHANDONG AGRICULTURAL UNIVERSITY
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AI Technical Summary

Problems solved by technology

However, so far, the dl gene on the Pp02 chromosome has not been identified, and the molecular markers closely linked to the peach double flower have not been developed, and it is difficult to achieve early molecular identification of the peach double flower

Method used

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  • TE molecular marker closely linked with peach double petals and application thereof
  • TE molecular marker closely linked with peach double petals and application thereof
  • TE molecular marker closely linked with peach double petals and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0028] 1. Development of double flower gene markers in natural variety populations

[0029] In order to solve the problem of obtaining accurate phenotype-associated sites, the present invention combined the high-depth resequencing data (average depth 20.3X) of 417 cultivated peach varieties covering each major production area for structural variation (SVs) detection, and screened out SVs sites~ 64,000. Using genome-wide association analysis (GWAS), it was found that a TE located at 25,860,343bp of chromosome Pp02 in the peach genome was strongly related to double flowers, named FD-TE-25.86. Figure 4 at the box.

[0030] figure 1 The phylogenetic tree, PCA (principal component analysis), population structure and LD (linkage disequilibrium) reduction trend of 417 peach resources are shown in figure 1 The a in is the high-quality SNP variation data filtered based on the whole genome, and the phylogenetic tree constructed based on the proximity method. figure 1 b. is the PCA p...

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Abstract

The invention discloses a TE molecular marker closely linked with peach double petals and application thereof. The TE molecular marker closely linked with the peach double petals is determined by taking separated progenies of the peach double petals as materials. The molecular marker can be used for replacing a traditional phenotype observation judgment method, so that the character identification of the double petals can be realized in the early stage. The TE molecular marker of the invention not only lays a foundation for fine localization and molecular cloning of the peach double-petals gene, but also provides an efficient way for molecular marker-assisted breeding of a new peach variety with the double-petals gene.

Description

technical field [0001] The invention relates to a TE molecular marker closely linked with peach double flower and application thereof, and belongs to the technical field of molecular marker-assisted selection. Background technique [0002] Peach is one of the most economically important fruit tree species in the world, diploid (2n=16), due to its small genome (~250M) and relatively short childhood (2~3 years), it is regarded as a key factor in the evolution of Rosaceae. A valuable resource and an important model plant for functional genomics research (Shulaev et al., 2008). Peach is native to China, domesticated in China 4,000 years ago, and then passed to European countries through the Silk Road (Faust et al., 1995). In my country, peach is the third largest deciduous fruit tree, which is widely cultivated all over the country and is an important part of agricultural production. [0003] Double flowers with extra petals are an important artificially selected trait for the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12Q1/686C12N15/11
CPCC12Q1/6895C12Q1/686C12Q2600/13C12Q2600/156C12Q2535/101
Inventor 谭秋平李玲肖伟
Owner SHANDONG AGRICULTURAL UNIVERSITY
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