Bifidobacterium lactis strain RK-7782 with intestinal regulation function and application of bifidobacterium lactis strain RK-7782

A technology of RK-7782 and Bifidobacterium lactis, applied in the field of biological strains, can solve the problems of poor effect or poor resistance, etc., and achieve the effect of significant inhibitory ability and excellent ability to resist human gastric juice and intestinal juice

Pending Publication Date: 2021-12-31
河北一然生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the known probiotics have poor resistance to the strong acidic digestive juice of gastric juice and intestinal juice, and are easily killed when passing through the gastrointestinal tract. As a result, after eating yogurt products containing probiotics, they can really regulate the human digestive system Very little live bacteria and balancing effect, giving a no or poor experience of consuming probiotic yoghurt

Method used

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  • Bifidobacterium lactis strain RK-7782 with intestinal regulation function and application of bifidobacterium lactis strain RK-7782
  • Bifidobacterium lactis strain RK-7782 with intestinal regulation function and application of bifidobacterium lactis strain RK-7782
  • Bifidobacterium lactis strain RK-7782 with intestinal regulation function and application of bifidobacterium lactis strain RK-7782

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] 1. Sample collection: Beginning in December 2019 in the Municipal Infant and Child Health Care Hospital, an anonymous survey was conducted on the health status of infants and young children in the area and feces samples were collected from infants aged 3-8 months.

[0036] Fecal samples were soaked in anaerobic diluent, numbered, and stored in liquid nitrogen. After collecting a predetermined number of samples, transfer all samples to a dry ice incubator, store them at -80°C, and isolate and culture the strains within 7 days.

[0037] 2. Separation and screening method

[0038] (1) Fully stir and disperse the feces samples of healthy infants immersed in the anaerobic diluent to obtain a feces suspension, which is serially diluted 10 times with the anaerobic diluent, and poured into a plate using the NPNL-X-Gal selective medium. Cultivate in the anaerobic bag with an anaerobic gas generating bag at 37°C for 40-60 hours to isolate potential bifidobacteria;

[0039] (2) ...

Embodiment 2

[0048] The animal experiments and bacterial displacement experiments are described in detail below.

[0049] 1. Experimental material: Bifidobacterium lactis RK-7782

[0050] 2. Experimental method

[0051] (1) Animal experiment design:

[0052] SPF grade Balb / C mice, 6 to 8 weeks old, female. Raised in SPF animal room: temperature 25±2°C, humidity 45%±1%, 12h light / dark cycle, free access to water and food. The mice were randomly divided into 10 groups (10 mice in each group), grouped as follows:

[0053] In the gavage group (3 groups), each mouse was treated with 2×10 8 , 2×10 9 and 2×10 10 cfu / rat orally for 30 days (0.2 mL per mouse per day);

[0054] The control group was intragastrically administered with normal saline;

[0055] During the experiment, the general appearance, behavior, poisoning performance and death of the mice were observed every day, and the body weight and food intake of the mice were recorded every week.

[0056] (2) Statistical analysis The...

Embodiment 3

[0063] Evaluation of strain tolerance:

[0064] Tolerance was evaluated by comparing the B. lactis RK-7782 strain with the commercial B. lactis CP-9. The acid resistance test, bile salt resistance test and simulated artificial gastrointestinal fluid test were carried out on CP-9, and the tolerance was evaluated by plate counting.

[0065] According to the experimental results, the following conclusions can be drawn: Figure 1-2 shown.

[0066] Within 0-4h, the survival number of Bifidobacterium lactis RK-7782 increased by about 0.5 orders of magnitude under the condition of pH3, and the number of survivals of Bifidobacterium lactis RK-7782 decreased by about two orders of magnitude in 0.3% bile salt. In addition, in the low concentration (mass concentration) of 0.1% bile salt within 4 hours, the survival number of Bifidobacterium lactis RK-7782 only decreased by about 0.5 orders of magnitude.

[0067] From the above experiments, it can be proved that it is equivalent to or ...

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Abstract

The invention relates to bifidobacterium lactis, in particular to bifidobacterium lactis, which is preserved in China General Microbiological Culture Collection Center (CGMCC) on August 24, 2021, the address is Institute of Microbiology, Chinese Academy of Sciences, No.3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, the bifidobacterium lactis is classified and named as bifidobacterium lactis RK-7782, and the preservation number is CGMCC No. 23181. The bifidobacterium lactis has relatively strong gastric juice and intestinal juice tolerance, has a remarkable inhibition capability on enteropathogenic bacteria, is proved to be safe, can be used for preparing yoghourt, cheese, probiotic compounds, ready-to-eat lactic acid bacteria and the like, and can be used for remarkably improving the microenvironment of intestinal flora of a human body and enhancing the defense capability of a digestive system of the human body.

Description

technical field [0001] The invention relates to the technical field of biological strains, in particular to a bifidobacterium lactis strain RK-7782 with intestinal regulation function and application thereof. Background technique [0002] Probiotics are a class of active microorganisms that can improve the microecological balance of the host and exert beneficial effects on the human body. In a normal adult, the intestinal tract contains more than 1,000 species of microorganisms, which can be divided into three categories: native flora, conditional pathogens, and transit bacteria. The original flora is the intestinal dominant bacteria with immune regulation and other functions; the conditional pathogenic bacteria are harmless when the intestinal flora is balanced, but will be harmful to the human body under certain conditions; the number of harmful bacteria is small under normal circumstances, and the bacteria When the group is out of balance, it can cause disease. Constipa...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23C9/123A23C19/032A23L33/135C12R1/01
CPCA23L33/135A23C9/1234A23C19/0323A23V2400/531A23V2400/51
Inventor 杨玲徐波贾晓蒙齐世华路江浩鄢梦洁张彦位李思童高一芳何方
Owner 河北一然生物科技股份有限公司
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