SNP molecular marker related to pig backfat thickness and application thereof
A technology of molecular markers and pig backfat thickness, which is applied in recombinant DNA technology, microbial measurement/inspection, DNA/RNA fragments, etc., can solve the problems of large distance span and limited application, so as to reduce feed intake and speed up the breeding process , the effect of increasing the frequency
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Embodiment 1
[0029] (1) Experimental animals
[0030] The experimental pig group used in the present invention is the purebred American Duroc head of the Breeding Pig Branch of Wen's Food Group Co., Ltd., which is the core group of the Breeding Pig Branch.
[0031] In this experiment, a total of 3769 Duroc pigs were selected in this resource group. The pigs in this experiment had free access to food and water, and the entire feeding method and feeding conditions were always consistent, which is a conventional method.
[0032] Duroc pigs have a small number of litters, with an average of only 9 to 10 pigs in a large group, but they grow fast, have a high feed conversion rate, a high carcass lean meat rate, a high intramuscular fat content, and strong stress resistance. Domestic commercial pig production has established a relatively mature hybrid supporting system. Among them, the hybrid combinations of Du Dachang continue to maintain an absolute dominant position in my country's domestic a...
Embodiment 2
[0052] Example 2 Target DNA sequence amplification and sequencing
[0053] (1) Primer design
[0054] The DNA sequence of SEQ ID NO: 1 on pig chromosome 1 was downloaded from the Ensembl website (http: / / asia.ensembl.org / index.html). And use primer design software primer premier 6.0 to design primers, entrust Sangon Bioengineering (Shanghai) Co., Ltd. to synthesize primers. The DNA sequences of the designed primers are as follows:
[0055] P001-F: 5'-ATTGGTGCTTTTCCTTAGAAGTCTTTC-3',
[0056] P002-R: 5'-GTCTACACATCCAGGCTTTCTCC-3';
[0057] (2) PCR amplification
[0058] Add 1 μL of DNA template, 3.4 μL of double distilled water, 5 μL of 2×Tag PCR StanMix with Loading Dye, and 0.3 μL of primers P001-F and P002-R into a 10 μL reaction system. The PCR reaction conditions were as follows: 5 minutes of pre-denaturation at 94°C, 35 cycles of denaturation at 94°C for 30 s, annealing at 64.5°C for 30 s, extension at 72°C for 45 s, and finally 5 min at 72°C.
[0059] (3) DNA sequenc...
Embodiment 3
[0063] Example 3 SNP site g.135A>G effect analysis of molecular markers
[0064] According to Table 1 and figure 2 It can be seen that for the backfat thickness, the effect of the SNP site g.135A>G dominant allele (AA) significantly reduces the backfat thickness by 1.16 mm on average than the GG phenotype. The phenotypic correlation analysis results of growth rate and backfat thickness traits show that the age at 100kg body weight is positively correlated with backfat thickness, reaching a very significant level, that is to say, the younger the pig grows to 100kg body weight, the more backfat. The thinner the meat, the higher the lean meat rate, which will greatly increase the economic value of pork and create wealth for the enterprise. In the individuals with this SNP molecular marker, by optimizing the dominant allele (A) of the SNP in American Duroc, the economic benefits of commercial pigs can be finally improved, thereby increasing the income of the enterprise.
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