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A Molecular Marker of Copy Number Variation on Pig Chromosome 13 Affecting Market Weight Age and Daily Gain and Its Application

A copy number and copy number increase technology, applied in the field of molecular biotechnology and molecular markers, can solve the problems of affecting gene expression, gene dosage effect, changing gene structure, etc., to improve growth performance, shorten the age, and speed up the breeding process. Effect

Active Publication Date: 2022-06-24
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is precisely because of the difference in the type of copy number variation of the fragment that it may lead to a dosage effect of the gene or change the structure of the gene, thereby significantly affecting the expression of the gene and the corresponding phenotype controlled by it.

Method used

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  • A Molecular Marker of Copy Number Variation on Pig Chromosome 13 Affecting Market Weight Age and Daily Gain and Its Application
  • A Molecular Marker of Copy Number Variation on Pig Chromosome 13 Affecting Market Weight Age and Daily Gain and Its Application
  • A Molecular Marker of Copy Number Variation on Pig Chromosome 13 Affecting Market Weight Age and Daily Gain and Its Application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] (1) Experimental animals

[0045] The experimental pig herd group used in the present invention is 3770 purebred American Duroc heads of the breeding pig branch of Wen's Food Group Co., Ltd., which is the core group of the breeding pig branch.

[0046] In this experiment, a total of 3770 American Duroc breeding pigs were selected from this resource group. The pigs had free access to food and water. The whole feeding method and feeding conditions were always consistent, which was a conventional method.

[0047] (2) Sample collection

[0048] The above-mentioned piglet docked tail and ear tissues were collected, soaked in 75% ethanol solution by volume, and stored in a -20°C refrigerator for later use.

[0049] (3) Types of 50K SNPs in the whole pig genome

[0050] The whole genome DNA was extracted from the ear tissue or tail tissue collected from each individual of the 3770 Duroc breeding pigs selected from the above resource groups, and the whole genome DNA was extra...

Embodiment 2

[0063] Example 2 Amplification and sequencing of target DNA sequences

[0064] (1) Primer design

[0065] From the NCBI website (http: / / www.ncbi.nlm.nih.gov), the specific DNA sequence (SEQ ID NO: 1) located in the 206578011bp-208240759bp sequence on chromosome 13 of pigs was downloaded as the reference sequence, and used Primer design software primer premier 6.0 was used to design qPCR primers (primer pair P1). At the same time, using the porcine GCG gene sequence (NC_010457.5) published by NCBI as the reference sequence, the qPCR primers (primer pair P2) were designed by the same method. The DNA sequences of the designed primer pairs P1 and P2 are shown below.

[0066] Primer pair P1 is:

[0067] Upstream primer F1: 5'-ACCTGGTGGCTGTGGCTGAG-3',

[0068] Downstream primer R1: 5'-CTGGTGCTGCTGTGGCTGTG-3';

[0069] Primer pair P2 is:

[0070] Upstream primer F2: 5'-TGCAGAACTACAGGGCTCAGGAC-3',

[0071] Downstream primer R2: 5'-GTGGAACAGGGCAGGTGGAAAG-3';

[0072] (2) qPCR a...

Embodiment 3

[0084] Example 3 Copy number type effect analysis of copy number variation molecular markers

[0085] According to Tables 1 and 2, the effect of the dominant copy number type (copy number gain) in the CNV region was significantly shortened by 6.36 compared with the average value of the copy number deletion type phenotype for the age of market weight. For daily gain, the effect of the dominant copy number type (copy number gain) in the CNV region was significantly higher by 26.42 than the mean value of the copy number deletion type. Therefore, through molecular marker-assisted selection, gradually culling pigs with deletion of copy number type in the population can significantly increase the frequency of copy number gain type and normal type, shorten the market weight and age of the population, and improve the daily weight gain of the population. , so that the growth and production performance of pigs are improved, and more high-quality commercial lean pork can be produced to m...

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Abstract

The invention belongs to the field of molecular biotechnology and molecular marker technology, and specifically relates to a copy number variation molecular marker on pig chromosome 13 that affects market weight age and daily weight gain and its application. The copy number variation region of the copy number variation molecular marker affecting market weight age and daily gain on pig chromosome 13 corresponds to the segment located in the interval 206578011bp-208240759bp on chromosome 13 of the International Porcine Genome Version 11.1 Reference Sequence. Copy number variation is prone to occur in the interval. By optimizing the dominant copy number variation type of the copy number variation molecular marker, the present invention can increase the frequency of the dominant copy number variation type generation by generation, shorten the age of weight on the market, increase the daily weight gain, and coordinate breeding of excellent breeding pigs with the above two traits , to speed up the progress of pig genetics and breeding so as to effectively improve the economic benefits of pig breeding.

Description

technical field [0001] The invention belongs to the technical field of molecular biotechnology and molecular markers, and in particular relates to a copy number variation molecular marker on chromosome 13 of pigs that affects the age and daily gain of market weight and its application. Background technique [0002] Pig is an important economic animal and a model animal for disease research. Some important economic traits of pig, such as growth, reproduction and meat quality traits, have always been concerned by breeding researchers. In recent years, with the continuous growth of people's pork consumption, lean pigs have occupied a large market share in the pork consumption market. Therefore, the selection of high-quality lean pigs has become the focus of breeding work, and the related traits that affect growth are important breeding goals. The growth traits of pigs mainly include market weight, age, and daily weight gain. Among them, the age of listed weight refers to the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 吴珍芳杨杰邱益彬丁荣荣蔡更元郑恩琴庄站伟吴杰李紫聪顾婷徐铮
Owner SOUTH CHINA AGRI UNIV
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