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Tissue culture industrialized seedling raising method for tilia miqueliana

A factory-based, tissue culture technology, applied in horticultural methods, botanical equipment and methods, horticultural tools/equipment, etc., can solve problems such as efficient factory production, no plant regeneration, etc., to improve production efficiency and optimize the cultivation cycle. , good effect

Inactive Publication Date: 2021-11-09
苏州回文生物种业科技有限公司
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0005] Although the invention realizes the tissue culture propagation of linden tree through four steps, it still uses "explants" for subculture in the implementation steps, and does not realize plant regeneration and efficient industrial production

Method used

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  • Tissue culture industrialized seedling raising method for tilia miqueliana
  • Tissue culture industrialized seedling raising method for tilia miqueliana
  • Tissue culture industrialized seedling raising method for tilia miqueliana

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Embodiment Construction

[0028] Reagent abbreviations: 6-BA is 6-benzylaminopurine; NAA is naphthaleneacetic acid; IBA is 3-indolebutyric acid; IAA is indole-3-acetic acid.

[0029] The selection of explant: select robust linden tree, use the full newborn branch of bud body as explant;

[0030] Disinfection of explants: first rinse the explants with tap water for 30 minutes, and rinse the surface; cut the branches into small sections of 2-3 cm, put them in a container and rinse them again; soak them in 70% alcohol for 1 minute, and transfer them to an ultra-clean In the workbench, soak it with 0.1% mercuric chloride solution for 8 minutes, and finally soak it with 0.15% sterile citric acid water for 3 times, dry it and inoculate it on the starting medium.

[0031] Start culture: inoculate on the start medium (medium D-1), insert 1 explant stem segment into each test tube. After 35 days of cultivation, the sterile vaccine can be obtained. Temperature: 25±3°C; light intensity: 1500-2100lux; light cult...

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Abstract

The invention belongs to the field of plant cultivation, and relates to a tissue culture seedling raising method, in particular to a rooting and seedling raising method for tilia miqueliana. The rooting and seedling raising method for tilia miqueliana comprises the following steps that (1), a sterile explant is prepared; (2), sterile seedlings are prepared; (3), tissue culture seedlings are prepared; (4), rooting seedling tissue culture seedlings are prepared; and (5), seedling hardening is carried out. According to the rooting and seedling raising method for tilia miqueliana, the culture period is short, and the total period of proliferation and rooting is about 10 weeks; the multiplication coefficient is high and can reach 1 : (4-4.5), the plant height after multiplication is about 3.5 cm, and the survival percent of pass after multiplication is greater than 98%; the rooting rate is 100%, and the survival rate after rooting is greater than 99%; and the survival rate after transplanting is greater than 98%, and the rooting and seedling raising method is suitable for industrialized seedling production of tilia miqueliana.

Description

technical field [0001] The invention belongs to the field of plant cultivation, and relates to a method for raising seedlings by tissue culture, in particular to a method for rooting and raising seedlings of Tilia cordata. Background technique [0002] Tilia Heartleaf (scientific name: Tilia cordata Mill.) is a plant of the genus Tilia in the family Tiliaceae, and the tree height can reach 35 meters in the wild state. Leaves are nearly round, 2-8 cm long, heart-shaped at the base. The flowers are light yellow, with 5 petals, fragrant, and the flowering period is from June to August. It is a high-quality nectar source tree species. Fruit subspherical, 4-8 cm in diameter, woody, gray-green, with inconspicuous ribs, densely tomentose, fruiting period August-September. Native to the limestone cliffs and wet woodlands of western England. The United States, France, Britain, Germany, the Netherlands and other countries have introduced and cultivated, and China's Xinjiang, Nanjin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/002A01H4/008
Inventor 张文杰尹翠翠冷江明郭俊清
Owner 苏州回文生物种业科技有限公司
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