Oocyte in-vitro maturation culture solution and application thereof

Abstract

The invention discloses a porcine oocyte in-vitro maturation culture solution added with a chemotactic factor ligand 14 (CXCL14). The culture solution can improve the quality of in-vitro maturation of oocytes. The quality of the oocytes is closely related to a parthenogenetic activation technology, an in-vitro fertilization technology and a somatic cell cloning technology, and improvement of the quality of the in-vitro mature oocytes plays an extremely important role in the fields of livestock production, medical reproduction, life science research and the like.

Description

technical field [0001] The invention relates to the technical field of cell culture, in particular to a culture medium for in vitro maturation of oocytes and an application thereof. Background technique [0002] In vitro matured oocytes can be used to prepare cloned embryos, in vitro fertilized embryos, parthenogenetically activated embryos, etc. The preparation of these embryos is closely related to many scientific researches, and has important uses in the fields of animal husbandry production, medical reproduction, and life science research. . However, in actual preparation, due to problems such as acquisition cost and technology, in vitro matured oocytes cannot be produced in large quantities and are of poor quality, so technologies such as somatic cell nuclear transfer cannot be widely used in various fields of scientific research and production. Somatic cell nuclear transfer technology is to transfer donor cells into enucleated in vitro mature oocytes, so that the gene...

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Problems solved by technology

[0003] Due to the influence of internal environment and many factors in the process of oocyte maturation in vivo, the core factors affecting oocyte maturation are not yet known

Therefore, in the in vitro maturation culture of oocytes, due to the lack of some unknown essential components, the quality of in vitro matured oocytes is much lower than that of normal matured oocytes in vivo, which limits the development of somatic cell nuclear transfer embryos, in vitro fertilized embryos, and parthenogenetic embryos. Efficient Production Application of Embryos

At present, it is generally believed that the main reason for the poor quality of in vitro matured oocytes is the lack of substances that scavenge free radicals and some factors necessary for maturation during in vitro maturation.

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