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Application of a biomarker in the screening and diagnosis of precocious puberty in children

A biomarker, children's technology, applied in the field of detection and analysis

Active Publication Date: 2022-06-17
上海血液生物医药有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Moreover, changes in endogenous sex hormones in the body usually occur at the same time as or after the appearance of secondary sexual characteristics. For example, in existing studies, reports have analyzed the feasibility of measuring serum sex hormones in diagnosing precocious puberty in children (see http: / / www. starlunwen.org / lunwen / 1423.html), but changes in endogenous sex hormones in the body are usually the result of precocious puberty, not the cause, so for children with precocious puberty, the clinic is very passive
Because the cause of precocious puberty in children has not been clearly reported, and there is no analysis and description of the early internal state before precocious puberty in children, such a situation results in that only when clinical secondary sexual characteristics appear prematurely in children Only when the child's precocious puberty can be discovered, but this time is too late

Method used

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  • Application of a biomarker in the screening and diagnosis of precocious puberty in children
  • Application of a biomarker in the screening and diagnosis of precocious puberty in children
  • Application of a biomarker in the screening and diagnosis of precocious puberty in children

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 138

[0040] Embodiment 1 Determination of 58 hormones in serum of 38 children

[0041] The present embodiment provides a kind of 58 hormone content determination methods, and the steps are as follows:

[0042] Step 1: Collect serum samples according to standard methods, store at -80°C until before measurement, and thaw the samples before measurement to prepare for measurement;

[0043] Step 2: Take 250 microliters of serum sample, add 250 microliters of methanol, add 250 microliters of water, vortex centrifuge to precipitate proteins;

[0044] Step 3: Take 600 microliters of supernatant and load it on SPE plate (SPE plate model: Oasis PRiME HLBμElution 96plate);

[0045] Step 4: add a mixed solution of acetonitrile / water (volume ratio 1:9) for elution, then add 200 microliters of hexane to elute, and discard the eluent;

[0046] Step 5: add 30 microliters of methanol / acetonitrile (volume ratio 1:9) mixed solution, collect the eluate, add 30 microliters of water to the eluate to d...

Embodiment 2

[0071] Embodiment 2 Determination of 58 hormones in urine of 40 children

[0072] The present embodiment provides a kind of 58 hormone content determination methods, and the steps are as follows:

[0073] Step 1: Collect urine samples according to standard methods, store at -80°C until before measurement, and thaw the samples before measurement to prepare for measurement;

[0074] Step 2: Take 0.5 ml of urine sample, add 0.25 ml of phosphate buffer, add 12.5 μl of glucuronidase derived from Roman snail for enzymatic hydrolysis, incubate at 55°C for 1 hour, and cool to room temperature;

[0075] Step 3: Take 600 microliters of supernatant and load it on SPE plate (SPE plate model: Oasis PRiME HLBμElution 96plate);

[0076] Step 4: add a mixed solution of acetonitrile / water (volume ratio 1:9) for elution, then add 200 microliters of hexane to elute, and discard the eluent;

[0077] Step 5: add 30 microliters of methanol / acetonitrile (volume ratio 1:9) mixed solution, collect t...

Embodiment 1

[0095] According to the results of Examples 1 and 2, the data in Tables 5 and 6 below are temporarily used as reference values ​​for judging precocious puberty in children. It should be noted that the normal clinical value generally needs to be in accordance with the standard specifications formulated in the international reference range, such as EP28-A3C etc., usually need more than 1000-2000 samples, in the present invention, only the current data is used to give the reference value:

[0096] Table 5 Reference values ​​of serum samples for judging precocious puberty in children

[0097] Compound number Compound name Reference 15 Hasinad 0.507735 31 corticosterone 0.627328 32 cortisone 10.08267 33 Deoxycorticosterone 0.027505 34 Deoxycortisone 0.736734 38 17a-Hydroxyprogesterone 0.137664 45 clobetasone butyrate 2.691339 48 fludrocortisone acetate 0.646598 49 methylprednisolone acetate 0.304498 51 ...

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Abstract

The invention provides an application of a biomarker in the preparation of children's precocious puberty screening and diagnosis products; the biomarker includes halcinonide, corticosterone, cortisone, 11-deoxycorticosterone, 11-deoxycortisol , 17α‑hydroxyprogesterone, betasone butyrate, fludrocortisone acetate, methylprednisolone acetate, prednisolone acetate, hydrocortisone valerate, methyltestosterone, One or more of androstenediones. The present invention measures a plurality of children's samples, and the results show that specific compounds have significant differences between healthy children and precocious puberty children, and the P value of T test is less than 0.1, indicating that the contents of these compounds have significant differences. Therefore, these compounds with significant differences can be used as diagnostic markers for early screening and diagnosis of precocious puberty in children.

Description

technical field [0001] The invention relates to the technical field of detection and analysis, in particular to the application of a biomarker in screening and diagnosis of precocious puberty in children. Background technique [0002] Precocious puberty (PP) is a sexual developmental disorder characterized by the early onset of sexual maturity. In recent years, domestic and foreign research data have shown that the incidence of precocious puberty in children is on the rise. Precocious puberty has a serious adverse effect on the normal growth and development of children. Children with precocious puberty are often accompanied by premature bone growth, which is more likely to cause children to be low in height in adulthood. . In addition, studies have shown that the occurrence of precocious puberty in children is related to the occurrence of malignant tumors of the reproductive system. Precocious puberty has a significant impact on children's psychology and behavior, which ma...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/14G01N30/34A61K45/00A61P15/08
CPCG01N30/02G01N30/06G01N30/14G01N30/34A61K45/00A61P15/08
Inventor 谭晓杰赵镇文郏征伟廖承慧徐永威
Owner 上海血液生物医药有限责任公司
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