Application of nicotinic acid in preparation of drug for relieving mammary gland fibrosis of dairy cows through GPR109A receptors

Abstract

The invention discloses an application of nicotinic acid in preparation of a drug for relieving mammary gland fibrosis of dairy cows through GPR109A receptors. The application comprises the following steps that S1, GPR109A receptor expression difference conditions are analyzed in mammary glands of normal dairy cows and fibrosis-prone dairy cows through in-vivo and in-vitro experiments, and it is determined that the GPR109A receptors are highly expressed in the mammary glands of the dairy cows with mammary gland fibrosis; and S2, fibrosis phenotypes in mammary epithelial cells of the dairy cows are detected, specifically, the change conditions of TGF-beta 1, alpha-SMA, Collagen2 and E-cadherin in the mammary epithelial cells of the dairy cows are specifically and correspondingly detected after the nicotinic acid is relieved. According to the application of nicotinic acid in preparation of the drug for relieving mammary gland fibrosis of the dairy cows through the GPR109A receptors, a brand-new relieving strategy for the mammary gland fibrosis tendency of the dairy cows is formulated, and the relieving effect of nicotinic acid on the mammary gland fibrosis tendency of the dairy cows is comprehensively evaluated by collecting normal mammary gland tissues and mammary gland fibrosis mammary gland tissues, separating primary mammary gland epithelial cells of the dairy cows and utilizing a molecular biology method. Nicotinic acid can effectively reduce rise of fibrosis phenotypes in mammary gland epithelial cells, so that the purpose of preventing the occurrence of mammary gland fibrosis diseases is achieved.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to the application of nicotinic acid in the preparation of a drug for alleviating mammary gland fibrosis in cows through GPR109A receptors. Background technique [0002] Mammary gland fibrosis in dairy cows is a phenomenon in which a large amount of extracellular matrix accumulates in mammary gland tissue, and scar tissue hyperplasia leads to necrosis of functional parenchymal cells of the mammary gland and is filled with connective tissue. The inflammatory response is a major factor in initiating the progression of the fibrotic response. Mammary gland fibrosis in dairy cows is a chronic progressive disease, and different types of mammary gland diseases may develop into mammary gland fibrosis. Mastitis is a very important disease among mammary gland diseases, and the global economic loss caused by cow mastitis is as high as 300 billion yuan every year. The symptoms of mastitis ar...

AI Technical Summary

Problems solved by technology

Therefore, niacin, as a new type of anti-mammary gland fibrosis drug, has advantages that traditional drugs do not have, but the effect of niacin on the tendency of mammary gland fibrosis is still unclear. tendency

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