Method for judging aging degree based on gene mutation and DNA methylation characteristics

Abstract

The invention relates to a method for judging aging degree based on gene mutation and DNA methylation characteristics. The method comprises the following steps: S1, collecting DNA information of a sample in a previous time period and a later time period, and performing DNA methylation sequencing on the DNA information; s2, screening reads of the sequencing data, and performing data inspection to obtain DNA methylation information and mutation information in a corresponding time period; s3, determining DNA methylation difference sites and determining the mutation information of mutation at the 1bp position near CpG, then calculating the DNA methylation rate, if the detected DNA methylation rate is decreased by more than 30% or the DNA methylation rate of a later sample is lower than 10%, it is judged that the sample is a senescence lock, determining that the DNA is senescence lock; s4, then judging the aging degree through an aging lock, wherein the aging lock is a phenomenon of DNA methylation loss in a 1bp range near the CpG site. According to the invention, data characteristics of DNA mutation information and DNA methylation modification information on a genome can be utilized to judge the aging degree of an organism.

Description

Technical field [0001] The present invention relates to the field of genes, in particular, a method of determining a degree of aging based on gene mutation and DNA methylation feature. Background technique [0002] Single nucleotide mutations is a common form of biological individual cell mutations. It is often studied that somatic mutation will accumulate as age growth, which will further cause a decline in cellular function, resulting in aging. Its molecular mechanism generally believes that there are two kinds, one is the mismatch in the gene replication, which is not fixed in time, resulting in cumulating the mutant base in the body cell development process, and the other case is cellopopyrimidine in demethylation Due to the transformation of cytosine due to deaminaceous action during the process of deamination becomes a thymidine. In this step, the AID / APOBEC gene can be converted to a T thymine (MC) in the DNA double strand in the DNA double strand. In the animal, the DME...

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Problems solved by technology

Abnormal DNA methylation will lead to changes in chromatin conformation, further resulting in the inability to transcribe the corresponding RNA or protein inactivation

[0004] Gene mutations and changes in DNA methylation modification are important factors that cause the aging of the body, tissue or somatic cells, but it is impossible to objectively and accurately determine the degree of aging in the prior art

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