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Differential gene analysis method based on BD single cell transcriptome and proteome sequencing data

An analysis method, a technology for sequencing data, used in sequence analysis, proteomics, genomics, etc.

Pending Publication Date: 2021-10-01
哈尔滨星云医学检验所有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is currently no complete set of processes that can combine single-cell transcriptome and proteome sequencing data for joint analysis, so there is an urgent need for a complete set of bioinformatics analysis methods based on BD single-cell transcriptome and proteome sequencing data Construct

Method used

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  • Differential gene analysis method based on BD single cell transcriptome and proteome sequencing data
  • Differential gene analysis method based on BD single cell transcriptome and proteome sequencing data

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Embodiment Construction

[0032] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without creative efforts fall within the protection scope of the present invention.

[0033] The present invention proposes a differential gene analysis method based on BD single-cell transcriptome and proteome sequencing data, which specifically includes the following steps:

[0034] S1. Obtain the sample library of BD single-cell transcriptome sequencing and proteome sequencing, and the sample library includes multiple samples.

[0035] Among them, BD single-cell transcriptome and proteome sequencing rely on the BD platform. BD single-cell transc...

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Abstract

The invention discloses a differential gene analysis method based on BD single cell transcriptome and proteome sequencing data, and belongs to the technical field of transcriptome data analysis. In order to solve the problem that no complete data analysis method can combine single cell transcriptome and proteome sequencing data for analysis in the prior art, differential gene analysis of cell subset classification in transcription and protein levels is carried out through a single cell transcriptome and proteome sequencing technology, cell characteristics are represented, and a biological mechanism transcribed to a protein regulation expression process is obtained, and a more accurate and richer BD single cell transcriptome and proteome sequencing data combined analysis method is provided. According to the analysis method, deep understanding of differential expression can be obtained, key mRNA or protein regulated after transcription is excavated, some important regulatory pathways are found and verified, and the analysis method can be applied to the field of tumors.

Description

technical field [0001] The invention belongs to the technical field of transcriptome data analysis, and in particular relates to a differential gene analysis method based on BD single-cell transcriptome and proteome sequencing data. Background technique [0002] BD single-cell transcriptome is a new technology for high-throughput sequencing of mRNA at the single-cell level. A small amount of transcriptome mRNA in a single cell isolated from multicellular organisms is efficiently amplified and then subjected to high-throughput sequencing. BD Single-cell transcriptome sequencing can effectively solve the problem of cell heterogeneity that cannot be cracked in tissue samples, help to discover new rare cell types, and gain an in-depth understanding of the expression regulation mechanism during development to reflect the heterogeneity between cells. Developmental biology, microbiology, neuroscience and other fields play an important role and are becoming the focus of life science...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G16B20/00G16B25/00G16B30/00G06K9/62
CPCG16B20/00G16B25/00G16B30/00G06F18/23
Inventor 刘志岩郭方郑青松
Owner 哈尔滨星云医学检验所有限公司
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