Method for separating tectoridin and tectorigenin from water extraction residues of rhizoma iridis tectori

A separation method and irisin technology, which are applied in chemical instruments and methods, preparation of sugar derivatives, sugar derivatives, etc., can solve simultaneous separation and preparation of shegan glycosides and irisin, waste of medicinal materials, increased process and cost input, etc. question

Active Publication Date: 2021-09-24
康美华大基因技术有限公司
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  • Application Information

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Problems solved by technology

This leads to a waste of medicinal materials
On the other hand, in order to prevent the dregs from re-flowing into the market, enterprises need to destroy the dregs, which increases the pr

Method used

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  • Method for separating tectoridin and tectorigenin from water extraction residues of rhizoma iridis tectori
  • Method for separating tectoridin and tectorigenin from water extraction residues of rhizoma iridis tectori
  • Method for separating tectoridin and tectorigenin from water extraction residues of rhizoma iridis tectori

Examples

Experimental program
Comparison scheme
Effect test

preparation example 1～14

[0061] Take 100g of Chuanshegan water-extracted medicinal residues, add a solvent, and extract; filter the extract, recover the solvent, and obtain Chuanshegan water-extracted medicinal residues extract. The specific parameters are shown in Table 1, and the dry paste yield is shown in Table 1.

[0062] Table 1

[0063]

[0064] It can be seen from Table 1 that methanol, methanol aqueous solution, ethanol, and ethanol aqueous solution can all be used for the extraction of Chuanshegan water-extracted medicinal residues, and 70vol% ethanol aqueous solution has the best extraction effect. The yield of dry ointment increases with the increase of the amount of solvent. When the amount of solvent reaches 5 times of the quality of Chuanshe dry water extraction residue, the increase of the yield of dry ointment tends to be flat. Considering the solvent recovery and cost in the later stage, the solvent The dosage is preferably 5 times of the quality of Chuanshegan water-extracted me...

preparation example 15

[0066] Take 5 kg of Chuanshegan water-extracted medicinal residues, add 25kg of 70vol% ethanol aqueous solution, heat and reflux for extraction for 1 hour; filter the extract, recover the solvent, and obtain 0.4 kg of Chuanshegan water-extracted medicinal residues.

Embodiment 1～8 and comparative example 1

[0068] Sephadex LH-20 chromatographic column (Sephadex LH-20 filler 900g), the ratio of diameter and height is shown in Table 2, the Sephadex LH-20 filler is swelled with mobile phase, and packed into 9 columns each containing 100g of filler. Get 18g of the Chuanshegan water-extracted medicinal residue extract obtained in Preparation Example 15, dissolve the Chuanshegan water-extracted medicinal residue extract in the mobile phase, and load the sample. slag extract), eluted with mobile equivalence, a total of 6 column volumes were eluted, the eluate was collected, the separation of the color bands was visually observed, and the components containing radixanthin and irisaxanthin were detected by TLC, and the crude radixanthin was obtained by combining And crude irisaxanthin.

[0069] Table 2

[0070]

[0071] If the diameter-to-height ratio is too high, the components of sheganoside and irisaxanthin cannot be completely separated. With the decrease of the diameter-height ra...

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Abstract

The invention discloses a method for separating tectoridin and tectorigenin from water extraction residues of rhizoma iridis tectori, which comprises the following steps of: loading a hydroxypropyl sephadex chromatographic column with a water extraction residue extract of rhizoma iridis tectori, eluting the hydroxypropyl sephadex chromatographic column, and collecting eluent to respectively obtain a tectoridin crude product and an tectorigenin crude product; wherein the diameter-height ratio of the hydroxypropyl sephadex chromatographic column is less than or equal to 1: 25. According to the invention, the tectoridin and the tectorigenin can be simultaneously separated from the water extraction residues of the rhizoma iridis tectori.

Description

technical field [0001] The invention relates to a method for separating sheganoside and irisaxanthin from Chuanshegan water extraction medicine dregs. Background technique [0002] Chinese medicine formula granules are granules made from single Chinese medicine decoction pieces. Under the guidance of the theory of traditional Chinese medicine, according to the formula of the clinical prescription of traditional Chinese medicine, it is used by patients for brewing. The "Technical Requirements for Quality Control and Standard Formulation of Traditional Chinese Medicine Formula Granules (Draft for Comment)" issued by the State Drug Administration stipulates that the preparation of traditional Chinese medicine formula granules, except for the molding process, should be basically the same as the traditional decoction, that is, water It is extracted by solvent, and produced by physical methods such as solid-liquid separation, concentration, drying, and granule molding. The effec...

Claims

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Application Information

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IPC IPC(8): C07H1/08C07H17/07C07D311/40C07D311/36
CPCC07H1/08C07H17/07C07D311/40C07D311/36
Inventor 李宁王丽胡星园韩丽娟杨学慧杨晓彬周建苗
Owner 康美华大基因技术有限公司
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