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Grapefruit oil with cowpea aphid insecticidal activity as well as insecticide and application thereof

A technology of insecticidal activity, grapefruit oil, applied in insecticides, applications, biocides, etc.

Active Publication Date: 2021-09-24
GANNAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Literature research shows that there are no related reports about the activity of grapefruit seed oil to kill cowpea aphids and its active chemical components. The present invention has obtained a botanical pesticide that has a significant insecticidal effect on cowpea aphids, and provides green and environmental protection for agricultural control of cowpea aphids. new type of insecticide

Method used

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  • Grapefruit oil with cowpea aphid insecticidal activity as well as insecticide and application thereof
  • Grapefruit oil with cowpea aphid insecticidal activity as well as insecticide and application thereof
  • Grapefruit oil with cowpea aphid insecticidal activity as well as insecticide and application thereof

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preparation example Construction

[0030] The invention provides the preparation method of the pomelo oil with the insecticidal activity of cowpea aphid, comprising the following steps:

[0031] 1) shredding pomelo seeds, soaking them in an aqueous ethanol solution with a volume concentration of 85% to 100%, heating and refluxing the obtained soaking solution to obtain an extracting solution;

[0032] 2) removing the solvent of the extract to obtain an extract;

[0033] 3) suspending the extract in water, extracting the suspension aqueous solution of the extract with petroleum ether, collecting the petroleum ether layer, removing the solvent, and obtaining pomelo oil.

[0034] In the invention, shredded pomelo seeds are soaked in an ethanol aqueous solution with a volume concentration of 85% to 100%, and the obtained soaking solution is heated and refluxed for extraction to obtain an extract.

[0035] The present invention has no special limitation on the source of grapefruit seeds, and the grapefruit seeds of...

Embodiment 1

[0049] The preparation method of grapefruit oil

[0050] 1. Types and sources of materials:

[0051] Shatian pomelo, a citrus belonging to Rutaceae, was picked from an orchard in Nankang District, Ganzhou City, Jiangxi Province in September 2019, and then the peel and pulp were removed, and the pomelo seeds were selected, and then the pomelo seeds were naturally air-dried and weighed to 502.395g before using scissors Shred and store in a 4°C refrigerator for later use.

[0052] 2. Extraction process of grapefruit oil:

[0053] Extraction of the total extract: Put 250g of shredded pomelo seeds into a 1000mL round-bottomed flask, add 300mL of 95% ethanol aqueous solution to soak for one night, then heat and reflux at 80°C for 2 hours, pour the extract into a clean round-bottomed flask Use a rotary evaporator to concentrate under reduced pressure at 45°C until all ethanol is removed to obtain an extract. Add 300mL 95% ethanol aqueous solution in the pomelo seed that has reflux...

Embodiment 2

[0068] The preparation method of grapefruit oil

[0069] 1. Types and sources of materials:

[0070] Shatian pomelo, a citrus belonging to Rutaceae, was picked from an orchard in Nankang District, Ganzhou City, Jiangxi Province in September 2019, and then the peel and pulp were removed, and the pomelo seeds were selected, and then the pomelo seeds were naturally air-dried and weighed to 502.395g before using scissors Shred and store in a 4°C refrigerator for later use.

[0071] 2. Extraction process of grapefruit oil:

[0072] Extraction of the total extract: Put 30g of shredded pomelo seeds into a 100mL round-bottomed flask, add 35mL of 90% ethanol aqueous solution to soak overnight, then heat and reflux at 82°C for 2.5h, pour out the extract into a clean round-bottomed flask Use a rotary evaporator to concentrate under reduced pressure at 45°C until all ethanol is removed to obtain an extract. Add 30mL 90% ethanol aqueous solution in the pomelo seed that has been refluxed...

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Abstract

The invention provides grapefruit oil with cowpea aphid insecticidal activity as well as an insecticide and application thereof, and belongs to the technical field of biological insecticides. The grapefruit oil provided by the invention contains 21 main active substances, and the contents of D-limonene, (Z, Z)-2, 4-decadienal, palmitic acid and linoleic acid are high (the relative content reaches 10% or above). The results of contact toxicity and stomach toxicity experiments of the cowpea aphids show that the grapefruit oil has very strong insecticidal activity on the cowpea aphids, and the mortality rates of the cowpea aphids respectively reach 96.5% and 93.3% after the grapefruit oil acts for 72 hours when the contact toxicity concentration is 2.0 micrograms per insect or the stomach toxicity concentration is 20 mg / mL. Meanwhile, the grapefruit oil is a plant secondary metabolite extracted from grapefruit by-product grapefruit seeds, belongs to a natural active substance, has the characteristics of greenness, environmental friendliness, low toxicity and the like, does not influence human health, and is a novel environment-friendly insecticide.

Description

technical field [0001] The invention belongs to the technical field of biological insecticides, and in particular relates to grapefruit oil with insecticidal activity against cowpea aphids, insecticides and applications thereof. Background technique [0002] Pests cause widespread damage to food crops such as grains and legumes by directly eating plant parts such as sprouts, seeds, flowers and plant sap. Pests feed on plants and their waste accumulation is detrimental to the plants it eats and humans. At the same time, pests lead to poor crop quality and cause huge economic losses to farmers. It has been reported that nearly 5% to 30% of the total global agricultural production is infected by insect pests every year. [0003] The cowpea aphid or black bean aphid is found all over the world and is especially common in temperate and tropical regions. It is a very harmful polyphagous pest that feeds on more than 80 plant families, with legumes being the preferred species. At ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N27/00A01N35/02A01N37/02A01N37/06A01N65/36A01P7/04
CPCA01N27/00A01N35/02A01N37/02A01N37/06A01N65/36Y02A50/30
Inventor 张军凌微张婧怡钟八莲
Owner GANNAN NORMAL UNIV
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