Cell centrifuging device and cell centrifuging, cleaning and culturing method

Abstract

The invention provides a cell centrifuging device and a cell centrifuging, cleaning and culturing method. The cell centrifuging device comprises a centrifugal cup and one or more liquid pushing plates, wherein the centrifugal cup is provided with a rotation axis and is used for being connected with a rotation driving mechanism and rotating around the rotation axis under the driving of the rotation driving mechanism so as to be used for centrifuging a blood sample contained in the centrifugal cup; the one or more liquid pushing plates are arranged in the centrifugal cup; and under the condition that the centrifugal cup rotates, the liquid pushing plates are used for pushing the blood sample to synchronously rotate in the rotating direction which is the same as the rotating direction of the centrifugal cup. According to the cell centrifuging device and the cell centrifuging, cleaning and culturing method provided by the invention, the liquid pushing plates are arranged in the centrifugal cup, and the blood sample in the centrifugal cup rotates synchronously with the centrifugal cup under the pushing action of the liquid pushing plates in the centrifugal cup process, so that cells in the blood sample are prevented from being sheared in the centrifugal process, and shear damage to the cells in the centrifugal process is reduced.

Description

Technical field [0001] The present invention relates to the field of medical devices, and in particular, to a cell centrifugal apparatus and cell centrifugal, cleaning and culture. Background technique [0002] In the cellular medical field, the cells collected by the living body often need to be cultured in the patient, in order to ensure the treatment effect of cells collected and cultured from the living body, the cells are sorted by peripheral blood single nuclear cells (PBMC sort) At the same time, it is necessary to remove impurities and medium in the cell culture process, concentrate the cells to the cell concentration suitable for therapy. Among them, the method of peripheral blood single nuclear cell sorting is the density gradient centrifugal method, i.e., using different particles in blood samples, at a certain rate, and finally form a zone on different regions to achieve a sorting. Purpose. [0003] During centrifugation, the inner side wall of the centrifugal apparat...

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Problems solved by technology

[0004] The invention provides a cell centrifugation device and cell centrifugation, cleaning and culturing methods, which are used to solve the problem that existing centrifugation equipment easily causes shear damage to cells during the centrifugation process

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