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Application of 5'-monophosphate nucleotides and their mixtures in the preparation of medicines or foods for improving mitochondrial function

A monophosphate nucleotide and mononucleotide technology, which is applied in the field of two 5'-monophosphate nucleotides and their mixtures, can solve the problem that exogenous nucleotides have not been reported in senescent cell mitochondria.

Active Publication Date: 2022-07-01
陈玉松
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the role of exogenous nucleotides obtained by enzymatic hydrolysis in improving the mitochondrial function of aging cells

Method used

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  • Application of 5'-monophosphate nucleotides and their mixtures in the preparation of medicines or foods for improving mitochondrial function
  • Application of 5'-monophosphate nucleotides and their mixtures in the preparation of medicines or foods for improving mitochondrial function
  • Application of 5'-monophosphate nucleotides and their mixtures in the preparation of medicines or foods for improving mitochondrial function

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] 1. The two 5'-monophosphate nucleotides used in this example are adenosine 5'-monophosphate and disodium 5'-uridylate, respectively.

[0029] 2. Production process: 90% pure ribonucleic acid (RNA) is subjected to thermal denaturation → nuclease P1 enzymatic hydrolysis → ultrafiltration → adsorption on anion resin → step elution → nanofiltration desalination → thin film concentration → toner decolorization → crystallization →Dry made. The above two kinds of 5'-monophosphate nucleotides were detected respectively and used after passing the test.

Embodiment 2

[0031] 1. The 5'-monophosphate nucleotide mixture used in this example is a nucleotide mixture in the form of five 5'-mononucleotide or its sodium salt, and the nucleotide mixture is CMP 25.80wt% , AMP22.80wt%, UMP20.40wt%, GMP 30.20wt%, IMP 0.8wt% ratio mixing.

[0032] 2. The specific preparation method is as follows:

[0033] Five kinds of 5' mononucleotides or their sodium salts are detected respectively, and they are ready for use after passing the test;

[0034] Pass the five kinds of 5' mononucleotides or their sodium salts that have passed the quality inspection through a 60-mesh sieve for use;

[0035] Calculate and weigh the required amount of each single nucleotide sample according to the proportion, add all the samples and mix them together, and the mixing time is not less than 40 minutes. The obtained samples were stored at room temperature.

Embodiment 3

[0037] The 5'-monophosphate nucleotide mixture used in this example is a nucleotide mixture in the form of five 5'-mononucleotides or their sodium salts. The nucleotide mixture is CMP 78wt%, AMP 6wt% %, UMP 7wt%, GMP 7wt%, IMP 2wt% ratio mixed. The preparation method is the same as that of Example 2.

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Abstract

The present invention relates to the application of 5'-monophosphate nucleotides and mixtures thereof in the preparation of medicines or foods for improving mitochondrial function. 5'-monophosphate nucleotides are produced by enzymatic degradation of ribonucleic acid as raw materials, and 5'-monophosphate 5'-monophosphate and 5'-uridylate disodium are more than 99% pure. The 5'-nucleotide mixture includes : CMP 23-78%, AMP 6-44%, UMP 7-40%, GMP 7-51%, IMP is 0, or greater than 0 and not higher than 2.5%. The present invention finds that 5'-monophosphate nucleotides and mixtures thereof can improve H 2 O 2 Mitochondrial function in induced senescent fibroblasts.

Description

technical field [0001] The invention belongs to the field of medicine and health care, and relates to a nucleotide product and a new use thereof, in particular to two kinds of 5'-monophosphate nucleotides produced by enzymatic degradation using ribonucleic acid as a raw material and a mixture thereof in medicine or food the use of. Background technique [0002] A large body of research evidence in the past decade has demonstrated a causal link between mitochondrial dysfunction and major phenotypes associated with aging. Perturbation of mitochondrial homeostasis is an important feature of cellular aging. Since the theory of free radical aging and mitochondrial aging theory were put forward, mitochondria have become the focus of aging research. The free radical theory believes that the ROS generated continuously during the metabolic process triggers the DNA chain free radical reaction, which causes the denaturation and crosslinking of DNA, nucleic acid, protein and lipid, es...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/7076A61K31/7072A61P39/06A23L33/13
CPCA61K31/7076A61K31/7072A61P39/06A23L33/13A23V2002/00A23V2200/30
Inventor 李勇珠娜徐美虹陈玉松
Owner 陈玉松
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