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Primer group for gene group detection for medication guidance of anxiety patients, related application and corresponding kit

A technique of medication guidance and primer set, applied in the biological field, can solve the problems of low throughput, high Sanger detection time and cost, time-consuming and labor-intensive, etc., and achieve the effect of precise medication guidance

Pending Publication Date: 2021-08-13
上海恩元生物科技有限公司
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In addition, for the detection of marker sites, fluorescent quantitative PCR method is commonly used at present. Fluorescent quantitative PCR method is simple to operate, but the throughput is not high, time-consuming and labor-intensive; Sanger detection time and cost as the gold standard are relatively high

Method used

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  • Primer group for gene group detection for medication guidance of anxiety patients, related application and corresponding kit
  • Primer group for gene group detection for medication guidance of anxiety patients, related application and corresponding kit
  • Primer group for gene group detection for medication guidance of anxiety patients, related application and corresponding kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] This example specifically illustrates the corresponding primer sets and kits that can be used for the gene group for medication guidance for patients with anxiety disorders.

[0024] This embodiment provides a primer set for the detection of gene groups for medication guidance for anxiety patients. The primer set includes primers for detecting the variation involved in each gene in the gene group, wherein the above gene group includes the following genes: CYP2C9, CYP2C19, CYP3A4 , CYP3A5, NAT2, ABCB1, SCN1A, UGT1A4, and POLG.

[0025] Further, by classifying the functional effects of drugs after entering the human body, among the above genes, genes CYP2C9, CYP2C19, CYP3A4, CYP3A5 and NAT2 are drug metabolism genes; genes ABCB1, SCN1A and UGT1A4 are drug efficacy genes; genes HLA and POLG are Drug Toxicity Genes.

[0026] In addition, in this embodiment, the drugs targeted by the above-mentioned drug metabolism genes are selected from phenytoin, clobazam, diazepam, buva...

Embodiment 2

[0087] In this example, some cases of anxiety disorders (Table 8) were sampled by the first-generation sequencing method and the primer set provided in Example 1, the corresponding kit and the corresponding method, and the mutations in Table 2 were detected. The results are shown in Table 9.

[0088]

[0089]

[0090]

[0091]

[0092]

[0093] In Table 9, genotyping instructions take the A / G SNP allele type as an example:

[0094] A: Indicates that the test sample is AA homozygous;

[0095] AG: Indicates that the test sample is AG heterozygous;

[0096] G: Indicates that the test sample is GG homozygous

[0097] In Table 9, the results are graded as follows:

[0098] A. Conservative: Indicates that the obtained results are highly reliable (the instrument automatically interprets the typing results);

[0099] B. Moderate: Indicates that the obtained results are of medium reliability (the instrument automatically interprets the typing results);

[0100] C. A...

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Abstract

The invention provides a primer group for gene group detection for medication guidance of anxiety patients, related application and a corresponding kit, the primer group comprises primers for detecting variation related to each gene in the gene group, and the gene group comprises the following genes: CYP2C9, CYP2C19, CYP3A4, CYP3A5, NAT2, ABCB1, SCN1A, UGT1A4 and POLG.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a primer set for gene group detection for medication guidance of patients with anxiety disorders and its use in preparing corresponding kits and the corresponding kits. Background technique [0002] Mental health problems have become a major public health problem and a prominent social problem in China. In recent years, the prevalence of mental illness in my country has been increasing year by year. Statistics show that the number of patients with various mental illnesses in my country is more than 100 million, and the number of patients with severe mental disorders has exceeded 18 million. Mental illness has ranked first in the social burden of disease in my country, and it is expected to reach 1 / 4 by 2020. Drug treatment is currently the main clinical method for mental illness, but there are huge individual differences in drug efficacy and adverse reactions. Studies h...

Claims

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Application Information

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IPC IPC(8): C12Q1/6883C12N15/11
CPCC12Q1/6883C12Q2600/106C12Q2600/156
Inventor 孙家权任斌魏小元
Owner 上海恩元生物科技有限公司
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