Electroactive bacterium whole-cell moisture power generation device and application thereof
A power generation device and electroactive bacteria technology, applied in the direction of generators, electrical components, generators/motors, etc. that convert kinetic energy into electrical energy, can solve problems such as limiting practical applications, complicated manufacturing processes, and cumbersome assembly processes, and meet the conditions for power generation Unrestricted, simple preparation method, high power generation effect
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Embodiment 1
[0069] An electroactive bacteria whole cell moisture power generation device, comprising a porous top electrode, a bottom electrode and Geobacter sulfur reducing PCA.
[0070] Concrete preparation method comprises the following steps:
[0071] (1) Preparation of whole-cell microbial film:
[0072] Get the cultivated Geobacter sulfur-reducing PCA bacterial liquid, centrifuge at 6000rpm to obtain the sulfur-reducing Geobacter PCA bacterial cell, then resuspend it quickly with ultrapure water, repeat several times, and obtain the sulfur-reducing Geobacter PCA bacterial liquid (1g wet cell / 10mL bacteria solution). Add 100 milliliters of bacterial liquid dropwise into the mold, and dry at 20°C to obtain a whole-cell microbial film of Geobacter sulfurreducens PCA with a thickness of 20 microns.
[0073] Wherein, the mold in this embodiment is: a hollow mold constructed of conductive glass (the area of the hollow part is 10×10 mm).
[0074] (2) place the whole-cell microbial me...
Embodiment 2
[0076] In the embodiment of the present invention, a whole-cell moisture power generation device was prepared according to the same method as in embodiment 1. The difference between this embodiment and embodiment 1 is:
[0077] In this example, Shewanella MR-1 was used instead of Geobacter sulfurreducens PCA to prepare a whole-cell microbial film.
Embodiment 3
[0079] In the embodiment of the present invention, a whole-cell moisture power generation device was prepared according to the same method as in embodiment 1. The difference between this embodiment and embodiment 1 is:
[0080] The drying temperature of the whole-cell microbial film of Geobacter sulfurreducens PCA in this example was raised to 80°C.
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