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Preparation method of high-purity hypocrellin

A high-purity technology for oleocanthal, applied in quinone separation/purification, resistance to vector-borne diseases, organic chemistry, etc., can solve the problems of undisclosed purity of crystals, cannot be reused, and difficult to regenerate silica gel, and achieves production safety. The effect of improved performance, low cost and long service life

Active Publication Date: 2021-08-10
山东元核生物工程有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The raw materials used in the extraction of these methods are all natural bamboo yellow, and the obtained crystals have no disclosed purity, and the silica gel column is eluted with different proportions of organic solvents, and the steps are relatively complicated.
Another disadvantage is that the silica gel used in silica gel column chromatography is difficult to regenerate. Most of them are thrown away after being used once and cannot be reused.

Method used

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  • Preparation method of high-purity hypocrellin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The preparation method of high-purity hypocrellin comprises the following steps:

[0029] (1) Take the solid-state fermented product of bamboo yellow fungus as raw material, weigh 50g and put it in the extractor, add ethanol solution, heat reflux at 75°C to extract twice, add 500ml 95% v / v ethanol solution for extraction for 1 hour each time; combine the extraction solution, reclaiming ethanol to obtain 10.2 g of hypocretin crude extract. After testing, the purity of hypocretin in the crude extract is 4.5%.

[0030] The preparation method of the solid-state fermented product of bamboo yellow fungus:

[0031] ①Strain source: The strain was isolated from wild bamboo yellow by our unit, and has been preserved in the China General Microorganism Culture Collection Center with the preservation number CGMCC No.18808 and the preservation date is December 02, 2019.

[0032] ② Preparation of seed inoculum: the medium used is PDB medium. After the culture medium was sterilized,...

Embodiment 2

[0040] The difference between the high-purity hypocretin preparation method and Example 1 is that the column height of the macroporous adsorption resin column ADS21 is 30 cm. Other steps are all the same as in Example 1.

[0041] After testing, the purity of hypocrellin was 73.5%.

Embodiment 3

[0043] The difference between the high-purity hypocrellin preparation method and Example 1 is that a macroporous adsorption resin column D101 is used, with a column height of 30 cm and a diameter of 3 cm. Other steps are all the same as in Example 1.

[0044] After testing, the purity of hypocrellin was 53.2%.

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Abstract

The invention belongs to the technical field of bioengineering, and particularly relates to a preparation method of high-purity hypocrellin. The method comprises the following steps: adding an ethanol solution into a hypocrellin-containing raw material, and extracting to obtain a hypocrellin crude extract; adding water, ethyl acetate and a NaHCO3 solution into the hypocrellin crude extract to remove impurities, so as to obtain a hypocrellin extract; and adsorbing the hypocrellin extract with macroporous adsorption resin, and eluting with an ethanol solution to obtain the hypocrellin extract. According to the method, the hypocrellin ethanol extract is further subjected to impurity removal, macroporous adsorption resin is used for adsorption, an ethanol solution is used for elution, and the high-purity hypocrellin is prepared; and the whole preparation process is simple in step, a used adsorption column is long in service cycle, convenient to regenerate and reusable, and the method is simple in step, high in safety, low in cost and beneficial to industrial production.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a preparation method of high-purity hypocrellin. Background technique [0002] Bamboo yellow fungus is a fungus that specifically parasitizes on some bamboo twigs. Mainly distributed in southern my country (Yunnan, Sichuan, Fujian, Hunan, Jiangsu and other regions), and also distributed in Sri Lanka and Japan (Li Xiangmin, Gao Jian, Yue Yongde, etc. Studies on the systematics, biology and active components of bamboo yellow[J] . Forestry Science Research, 2009, 22(2):279.). Bamboo yellow is a kind of folk medicinal material, which can be used to treat diseases such as rheumatoid arthritis, cold stomach pain, sciatica, tracheitis, whooping cough, bruises and anemia headache (Zhong Shurong, Zhao Hai, Li Anming, etc. A kind is yet to be The developed Chinese medicine - Zhuhuang [J]. Chinese Herbal Medicine, 2002,33(4):372-374.). In recent years, scholars at home ...

Claims

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Application Information

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IPC IPC(8): C07C50/36C07C46/10
CPCC07C50/36C07C46/10C07C2603/54Y02A50/30
Inventor 袁建国田特刘代成
Owner 山东元核生物工程有限公司
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