Multi-drug resistance inhibitor screening method based on Raman spectrum

A technology for inhibitor screening and multidrug resistance, which can be used in Raman scattering, material analysis by optical means, measurement devices, etc.

Active Publication Date: 2021-07-06
SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But so far, the application of SERS in drug screening has not been published

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  • Multi-drug resistance inhibitor screening method based on Raman spectrum
  • Multi-drug resistance inhibitor screening method based on Raman spectrum
  • Multi-drug resistance inhibitor screening method based on Raman spectrum

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Embodiment Construction

[0026] The present invention will be further described in detail below in conjunction with the embodiments, so that those skilled in the art can implement it with reference to the description.

[0027] It should be understood that terms such as "having", "comprising" and "including" used herein do not exclude the presence or addition of one or more other elements or combinations thereof.

[0028] refer to figure 1 , a method for screening multi-drug resistance inhibitors based on Raman spectroscopy of the present embodiment, comprising the following steps:

[0029] S1. Preparation of SERS substrate

[0030] Specifically include:

[0031] S1-1. Pretreatment of silicon wafers: rinse the silicon wafers with absolute ethanol, dry them, and place them in H with a volume ratio of 3:1. 2 SO 4 and H 2 o 2 Wash in the mixed solution for 15 minutes, then fully rinse with deionized water and ultrapure water respectively, N 2 Blow dry; the cleaned silicon wafers were completely imm...

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Abstract

The invention discloses a multi-drug resistance inhibitor screening method based on Raman spectrum. The method comprises the following steps: 1) preparing an SERS substrate; (2) coupling multi-drug resistant protein on the SERS substrate; (3) randomly selecting a plurality of points on the SERS substrate coupled with the multi-drug resistant protein for Raman signal detection, and calculating the average Raman intensity value of the plurality of points as a Raman standard value; and 4) co-incubating a multi-drug resistance inhibitor to be detected with the SERS substrate, cleaning, detecting the Raman intensity of the SERS substrate, and comparing the Raman intensity with the Raman standard value, thereby judging the inhibition effect of the multi-drug resistance inhibitor to be detected. According to the method, the surface enhanced Raman spectrum is successfully applied to screening of the multi-drug resistance inhibitor, the high-sensitivity and rapid identification of the inhibition effect of the multi-drug resistance inhibitor can be achieved through Raman spectrum fingerprint detection, the requirement for the drug screening rate in the current pharmaceutical industry can be met, and the method has very high application and popularization value.

Description

technical field [0001] The invention relates to the field of drug screening, in particular to a method for screening multi-drug resistance inhibitors based on Raman spectroscopy. Background technique [0002] The multidrug resistance mechanism mediated by multidrug resistance protein (Pgp) is the main reason for the failure of chemotherapy and leads to the final death of more than 90% of chemotherapy patients. In view of this, screening Pgp inhibitors and reversing multidrug resistance has become a possible choice to enhance the effect of chemotherapy. So far, the development of Pgp inhibitors has gone through three generations, from known transporter substrates, substrate derivatives and combinatorial chemical design of inhibitors. However, no clinically available effective inhibitors have been screened so far. This is because, on the one hand, the non-specific toxicity of these inhibitors in the body is still unavoidable. More importantly, with the deepening of research ...

Claims

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Application Information

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IPC IPC(8): G01N21/65
CPCG01N21/658
Inventor 殷建尹焕才张志强陈名利
Owner SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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