Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Molecular marker for detecting deep notch marker character of rape leaf margin and application thereof

A technology related to molecular markers and rapeseed leaves, which is applied in the direction of microbial measurement/inspection, DNA/RNA fragments, recombinant DNA technology, etc., can solve the problem of successful gene transfer of the leaf edge deep notch trait of rapeseed, and achieve remarkable results. High light efficiency, remarkable resistance to dense planting, and the effect of overcoming low efficiency

Active Publication Date: 2021-06-11
江西省农业科学院作物研究所
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008]Therefore, the technical problem to be solved by the present invention is to overcome the defects in the prior art that it is impossible to confirm from the molecular level whether the gene of the leaf edge deep notch trait of rapeseed is successfully bred , so as to provide a molecular marker for detecting the deep notch marker traits of rapeseed leaf margin and its application, so as to speed up the breeding of rapeseed varieties

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Molecular marker for detecting deep notch marker character of rape leaf margin and application thereof
  • Molecular marker for detecting deep notch marker character of rape leaf margin and application thereof
  • Molecular marker for detecting deep notch marker character of rape leaf margin and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] 1. Construction of rapeseed with leaf margin deep notch marker traits and location of rapeseed leaf margin deep notch marker traits

[0036] Brassica oleracea Zhongshuang 11 was crossed with a wild plant, Rapeseed brassicae, and a rapeseed with deep notched leaf margins was obtained. The leaves with deep notches in rapeseed leaf margins were analyzed by full-length transcriptome sequencing (iso-seq), and the genes that were transferred from the saponinia genome to the rapeseed genome that caused the deep-cleft phenotype of rapeseed leaves were excavated. The specific process is:

[0037] A total of 92,897 full-length transcripts were detected in rapeseed with deep notch marks on leaf margins. Using the genome sequenced on Shuang11 in the parents as the reference genome, a total of 80,879 transcripts were compared to the reference genome. It was 87.06%, and the remaining 12018 transcripts were not aligned to the reference genome. The genomes of cabbage and cabbage were...

Embodiment 2

[0059] Example 2 Comparative Example of Molecular Markers of Leaf Margin Deep Notches Marking Traits

[0060] Using exactly the same steps as in Example 1 to obtain rapeseed with deep notch markers on leaf margins, and locate the transcripts of deep notch markers on rapeseed leaf margins, the cDNAs of transcripts that are not similar to Chinese cabbage, cabbage and rapeseed sequences were screened The sequence is shown in SEQ ID NO: 6, the nucleotide sequence translated from the transcript is shown in SEQ ID NO: 7, and the amino acid sequence of the translated protein is shown in SEQ ID NO: 8.

[0061] According to the sequence of SEQ ID NO: 6, a primer pair of the sequence is designed and synthesized, and the primer pair is composed of single-stranded DNA molecules shown in SEQ ID NO: 9 and SEQ ID NO: 10.

[0062] The sequence shown in SEQ ID NO: 6 was named LSD1, and the sequence shown in SEQ ID NO: 5 was named LSD2. The proteins translated from the above sequences were comp...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the technical field of detection of marker characters of oilseed rape, in particular to a molecular marker for detecting deep notch marker characters of leaf margin of oilseed rape and application thereof. The invention provides a primer pair. The primer pair is composed of single-stranded DNA molecules as shown in SEQ ID NO: 1 and SEQ ID NO: 2. The invention also discloses a PCR reagent, wherein the PCR reagent comprises the primer pair. The invention overcomes the defect that in the prior art, whether the gene with the leaf margin deep notch character of the rape is successfully transformed or not cannot be determined from the molecular level, so that the molecular marker for detecting the leaf margin deep notch marker character of the rape and the application of the molecular marker are provided, and the breeding of rape varieties is accelerated.

Description

technical field [0001] The invention relates to the technical field of detection of marker traits of rapeseed, in particular to a molecular marker for detecting marker traits of deep notches in rapeseed leaf margins and an application thereof. Background technique [0002] At present, more than 80% of my country's rapeseed production uses hybrid varieties, mainly three-line hybrids, and the traditional hybrid rapeseed seed production process of Brassica napus mainly has the following three problems: [0003] One is that the breeding process of CMS is easy to be confused. Specifically, during the breeding process of the CMS line, since the CMS line and the maintainer line are both cabbage-type, the appearance of the leaves and plants is very similar, and it is difficult to distinguish them after removal of impurities; The sterile maintainer line is left over, resulting in a decrease in the purity of the sterile line. [0004] The second is to reduce the output of seed produ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/156C12Q2600/13
Inventor 辛佳佳谭河林谷德平徐亮戴兴临张洋涂玉琴涂伟凤汤洁万鹏
Owner 江西省农业科学院作物研究所
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com