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Application of epigenetic modification OsMOF protein in improvement of rice yield traits

A protein and rice technology, applied in the fields of application, plant genetic improvement, plant products, etc., can solve the problems of unidentified members of apparent modification proteins and backward research

Active Publication Date: 2021-06-08
上海浦东复旦大学张江科技研究院 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In humans and mice, the research on the acetylation modification function of MOF on histone H4K16 is relatively mature, but in plants, especially in rice, the related research is relatively backward, and most of the members of the epigenetic modification proteins have not yet been identified and expressed. epigenetic modifier protein OSMO Reports on the Application of Genes in Improving Rice Yield Traits

Method used

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  • Application of epigenetic modification OsMOF protein in improvement of rice yield traits
  • Application of epigenetic modification OsMOF protein in improvement of rice yield traits
  • Application of epigenetic modification OsMOF protein in improvement of rice yield traits

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Example 1. Construction OSMO gene expression vector

[0053] (1) OSMO Gene and Protein Structural Composition

[0054] rice OSMO Gene, NCBI database Gene ID: 4343971. The gene is located on rice chromosome 7, with a total length of 4891bp in the transcribed region of the genome, NCBI number NC_029262.1, with 9 exons and 8 introns, and the transcription structure diagram is as follows figure 1 shown. The transcribed sequence is numbered XM_015789227.2 by NCBI. The full length of the sequence is 1655bp, and the full length of the coding region is 1353bp. The sequence is shown in SEQ ID NO.1. The gene encodes 450 amino acids, the OsMOF protein sequence NCBI number XP_015644713.1, and the amino acid sequence is shown in SEQ ID NO.2. The rice OsMOF protein contains 1 RNA binding activity-knot (RNA binding activity) functional domain and 1 N-Acyltransferase superfamily (acetyltransferase activity) functional domain. The structure of the protein functional domain is sho...

Embodiment 2

[0072] Example 2. OSMO Gene domain activity analysis

[0073] rice OSMO The sequence of two functional domains of the gene, RNA binding activity-knot (RNA binding activity) functional domain and N-Acyltransferase superfamily (acetyltransferase activity) functional domain:

[0074] SEQ ID NO.5: Rice OSMO Gene-encoded protein RNA binding activity-knot (RNA binding activity) functional domain amino acid sequence (60aa)

[0075] LEVGTRVMCRWRDQKLHPVKVIERRKSSTSSSPADYEYYVHYTEFNRRLDEWVKLEQLDL.

[0076] SEQ ID NO.6: Rice OSMO Amino acid sequence of functional domain of gene-encoded protein N-Acyltransferase superfamily (acetyltransferase activity) (284aa)

[0077] LDAASLREHEEFTKVKNIAKIELGRYEIDTWYFSPFPPEYNDSPKLFFCEFCLNFMKRKEQLQRHMKKCDLKHPPGDEIYRSGTLSMFEVDGKKNKVYGQNLCYLAKLFLDHKTLYYDVDLFLFYVLCECDDRGCHMVGYFSKEKHSEESYNLACILTLPPYQRKGYGKFLIAFSYELSKKEGKVGTPERPLSDLGLLSYRGYWTRVLLEILKKHKSNISIKELSDMTAIKADDILSTLQSLDLIQYRKGQHVICADPKVLDRHLKAAGRGGLEVDVSKLIWTP。

[0078] rice OSMO The two functio...

Embodiment 3

[0080] Example 3. OSMO Transformation of rice receptor with gene expression vector

[0081] The rice material is the indica variety Minghui 63, and the transformation recipient material is the callus induced by mature embryos of this variety. The plant medium used in the transformation process is MS basic medium. The main components and formula are shown in Table 1. The transformation method is an Agrobacterium transformation method, the Agrobacterium tumefaciens strain is Agrobacterium tumefaciens LBA4404, and the microbial culture medium used for cultivating the Agrobacterium in the transformation process is a YEP medium.

[0082] Table 1. Plant media for rice transformation

[0083]

[0084] Note: *The above media all contain 30g / L sucrose + 2.5g / L plant agar.

[0085] (1) Rice callus induction

[0086] ① Remove the hulls from the seeds of the mature rice indica variety Minghui 63.

[0087] ② Under aseptic conditions, soak with 70% ethanol for 5 minutes and wash with...

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PUM

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Abstract

The invention discloses application of epigenetic modification OsMOF protein in improvement of rice yield traits. The OsMOF protein is shown as any one of the following (1)-(3): (1) protein composed of amino acids shown as SEQ ID NO.2; (2) a protein which is derived from (1) and has the same function with the amino acid shown in SEQ ID NO.2 through substitution and / or deletion and / or addition of one or more amino acid residues; and (3) a protein or a derivative thereof which is generated from a homologous sequence and is derived from other varieties of rice or other species and has homology of at least 50% and has the same function as that of (1). The invention discloses the application of the endogenous epigenetic modification protein OsMOF of rice in improvement of rice yield traits, and proves that the gene can significantly improve the rice yield traits.

Description

technical field [0001] The invention belongs to the technical field of gene breeding, more specifically, relates to OSMO Application of genes in improving rice yield traits. Background technique [0002] Rice is one of the most important food crops in the world, and more than 3 billion people around the world depend on rice as their main ration. In view of environmental climate change and population expansion, it is estimated that rice production will need to increase by 40% to meet the demand by 2030, and rice production-increasing traits have always been a key area of ​​crop genetics and breeding. With the development of molecular genetics and genomics, more and more biological macromolecules have been identified, and the most cutting-edge new discoveries are used in molecular genetic breeding of rice to mine genetic resources for crop production and provide planting for future food security. reserve. [0003] In the first half of the 20th century, Waddington first prop...

Claims

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Application Information

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IPC IPC(8): C07K14/415C12N15/29C12N15/84A01H5/00A01H6/46
CPCC07K14/415C12N15/8261
Inventor 王莹杨金水胡子欣
Owner 上海浦东复旦大学张江科技研究院
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