A surface-enhanced Raman spectroscopy detection method for melamine and formaldehyde
A technology of melamine and detection method, applied in the field of chemical detection, can solve the problems of lack of reproducibility and selectivity of SERS analysis and detection, unable to meet the detection of actual complex matrix samples, etc., to improve the stability and storage time, and the migration method is simple and effective. , easy to store effects
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Embodiment 1
[0061] In this example, a formaldehyde SERS substrate is prepared, and the specific process is as follows:
[0062] S1: Preparation of silver nanoparticles: Measure 100mL10mmol / L silver nitrate solution into a 250mL three-neck flask, heat in an oil bath, keep magnetic stirring and condensing reflux during heating, after heating to boiling state, continue magnetic stirring, and quickly add 1 mL of 2% (w / w) sodium citrate solution was continued to reflux for 1 h under heating and stirring, stirred and cooled to room temperature, and its concentration was doubled by centrifugation (6000 rpm, 10 min) to obtain the desired silver nanoparticles.
[0063] S2: Preparation of formaldehyde SERS substrate: Mix the silver nanoparticles prepared in S1 with 0.2 mg / mL reduced graphene oxide at a ratio of 1:1 (v:v) and incubate in the dark for 12 h. Immediately, 5 mL of the above mixture was loaded on 13 mm chromatographic paper through a syringe, and then the chromatographic paper loaded wit...
Embodiment 2
[0065] In this example, a formaldehyde SERS substrate is prepared, and the specific process is as follows:
[0066] S1: Preparation of silver nanoparticles: Measure 100mL10mmol / L silver nitrate solution into a 250mL three-neck flask, heat in an oil bath, keep magnetic stirring and condensing reflux during heating, after heating to boiling state, continue magnetic stirring, and quickly add 1 mL of 2% (w / w) sodium citrate solution was continued to reflux for 1 h under heating and stirring, stirred and cooled to room temperature, and its concentration was doubled by centrifugation (6000 rpm, 10 min) to obtain the desired silver nanoparticles.
[0067] S2: Preparation of formaldehyde SERS substrate: Mix the silver nanoparticles (Ag) prepared in S1 with 0.2 mg / mL reduced graphene oxide (rGO) at a ratio of 1.2:1 (v:v) and incubate in the dark 12h. Immediately, 5 mL of the above mixture was loaded on 13 mm chromatographic paper through a syringe, and then the chromatographic paper l...
Embodiment 3
[0075] In this embodiment, a melamine SERS substrate is prepared, and the specific process is as follows:
[0076] S1: Synthesis of silicon spheres: Add 90 mL of absolute ethanol and 13.5 mL of ammonia water to a 250 mL round-bottomed flask, then add 9.0 mL of tetraethyl orthosilicate dropwise and stir for 24 hours. After cleaning for 3 times and drying, the silicon spheres with a diameter of about 430nm can be obtained.
[0077] S2: Synthesis of silicon spheres coated with silver nanoparticles: take 200 mg of silicon spheres prepared by S1 and disperse them in 20 mL of absolute ethanol, and ultrasonically disperse them for 30 min. Prepare 0.2mol / L silver ammonium solution with 5% ammonia water. Take 10mL of 0.2mol / L silver ammonium solution and add it to 20mL of absolute ethanol-dispersed silicon sphere solution, and stir for 1h. Take 1 g of polyvinylpyrrolidone with a molecular weight of 58000 and disperse it in 50 mL of absolute ethanol, then add the mixture of silicon sp...
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