Double-excitation orthogonal emission up-conversion luminescence nano-particles, multi-flux detection immunochromatography test paper and application of multi-flux detection immunochromatography test paper

Abstract

The invention discloses a double-excitation orthogonal emission up-conversion luminescent nanoparticle which is characterized in that the double-excitation orthogonal emission up-conversion luminescent nanoparticle is of a core-shell structure with two layers of shells, wherein a NaYF4 nanoparticle doped with Yb, Tm and Er is used as an inner core, a first shell layer is formed by Yb-doped NaYF4, a second shell layer is formed by by NaYF4 doped with Yb and Nd, and a Yb, Tm, Er and Nd co-doped NaYF4: Yb / Tm / Er (at) NaYF4: Yb (at) NaYF4: Yb / Nd core-shell nanoparticle structure is formed; the surface of the nano-particle is marked with an antigen of a detected object, and the nano-particle emits red light under the excitation of near-infrared laser with the wavelength of 980 nm and emits green light under the excitation of laser with the wavelength of 808 nm. The invention further discloses multi-flux detection immunochromatography test paper adopting the nano-particles and application of the multi-flux detection immunochromatography test paper. By synchronously improving the nanoparticle material, the test paper and the detection method, the detection operation steps are greatly simplified, the cost is low, the detection sensitivity is relatively high, and multi-flux, rapid, qualitative and quantitative detection of food can be realized.

Description

technical field [0001] The invention belongs to the technical field of biomedical diagnosis materials and detection, and in particular relates to a dual-excitation orthogonal emission up-conversion luminescent nanoparticle, a multi-flux detection immunochromatographic test paper and applications thereof. Background technique [0002] Among the most important problems affecting the quality and safety of grain and oil in the world, the pollution of grain and oil products by mycotoxins, heavy metals, and pesticide residues is the most serious. According to the statistics of the Food and Agriculture Organization of the United Nations (FAO), 25% of the food in the world is polluted by mycotoxins such as aflatoxin, and the loss of mildew reaches 1.5%-3% of the total output. Aflatoxin B1 is an important toxic mycotoxin that is always produced during grain growth and storage. Ingestion of AFB1 may cause serious diseases such as acute liver cirrhosis, cell necrosis, and canceration, ...

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Problems solved by technology

[0004] Accurate results and statutory approval are the biggest advantages of physical and chemical methods, but the equipment is expensive, the pretreatment process is complicated, the operation is cumbersome, the efficiency is low, and the cost is extremely high, so it is not suitable for large-scale sample screening and daily internal control testing. For final confirmation only

Enzyme-linked immunosorbent assay (ELISA) method and colloidal gold rapid detection card are commonly used rapid detection methods in enterprises, but both have their own advantages and disadvantages: ELISA method has relatively high sensitivity and quantitative detection, but the operation process is relatively complicated. The environment and personnel requirements are high, the environmental interference factors are complex, and the repeatability is poor; the traditional colloidal gold detection card can meet the rapid on-site requirements, and can be quickly measured within 5-10 minutes, but it can only be qualitative, and the sensitivity is low, and the naked eye is subjective. Strong reproducibility, poor reproducibility, and requires multiple antibody-modified AuNPs to be evenly immobilized on the same binding pad, which will lead to cross-reactivity between different AuNPs and test lines, and reduce detection accuracy

However, in actual application, the inventor found that the colloidal gold chromatography test strip can only detect the composition and concentration of one substance to be detected at a time, which cannot meet the rapid detection requirements for multiple substances in food, and is not suitable for use. The equipment performs automatic and multi-throughput detection, so it has the problems of slow detection speed and low efficiency, and further improvement is needed

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