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A kind of phospholipid microbead as artificial antigen presenting cell and its preparation method and application

A technology of artificial antigens and microbeads, applied in biochemical equipment and methods, animal cells, vertebrate cells, etc., can solve problems such as application limitations, difficulty in large-scale acquisition of DCs, and difficulty in controlling the differentiation state, to achieve increased interaction, Strong adsorption capacity, the effect of improving the amount and quality of T cell expansion

Active Publication Date: 2022-05-17
CARBIOGENE THERAPEUTICS CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it is difficult to control the differentiation state of DCs under in vitro culture conditions, and it is difficult to obtain DCs from patients' autologous sources on a large scale
In the context of the general trend of research and development of allogeneic universal cell therapy products, the application of DC is greatly limited

Method used

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  • A kind of phospholipid microbead as artificial antigen presenting cell and its preparation method and application
  • A kind of phospholipid microbead as artificial antigen presenting cell and its preparation method and application

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preparation example Construction

[0032] A method for preparing phospholipid microbeads as artificial antigen-presenting cells, comprising the steps of:

[0033] Prepare materials according to recipes;

[0034] Dissolving the agar in hot water, as a preferred method, the hot water is hot water at 60°C to form an agar solution, and then add gelatin to dissolve it;

[0035] Adding phosphatidylcholine and polyethylene glycol monomethyl ether to ethanol and stirring and mixing, as a preference, the rotation speed is 200 rpm to obtain the first mixed solution;

[0036] Mix the agar-gelatin mixed solution with an equal volume of organic solvent, stir, and as a preferred method, the rotating speed is 380 rpm to obtain the second mixed solution; as an example, the organic solvent includes: butyl acetate, chloroform, gasoline ; As a preference, it is better to use ethyl acetate as non-toxic.

[0037] Pour the first mixed solution into the second mixed solution, and stir, as a preferred method, at a speed of 380 rpm t...

Embodiment 1

[0040] Embodiment 1: 32g agar, 24g gelatin, 26g phosphatidylcholine, 28g magnetic powder, 10g polyethylene glycol monomethyl ether;

Embodiment 2

[0041] Embodiment 2: 36g agar, 20g gelatin, 30g phosphatidylcholine, 32g magnetic powder, 6g polyethylene glycol monomethyl ether;

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Abstract

The invention discloses a phospholipid microbead used as an artificial antigen-presenting cell and its preparation method and application. The formula of the phospholipid microbead includes: 32-36 parts of agar, 20-24 parts of gelatin, 26-30 parts Phosphatidylcholine, 28-32 parts of magnetic powder, 6-10 parts of polyethylene glycol monomethyl ether; preparation method, including: configuring agar-gelatin mixed solution; adding phosphatidylcholine and polyethylene glycol monomethyl ether in ethanol The ether surfactant is stirred and mixed; the agar-gelatin mixed solution is added to the organic solvent and then mixed with the surfactant, then the magnetic powder is added, stirred, and ice water is added to obtain the phospholipid microbeads; the phospholipid microbeads of the present invention have uniform adsorption force to nucleic acid , and has a strong adsorption capacity, which increases the contact opportunities between nucleic acids and nucleic acids, and between nucleic acids and cytokines, and can be applied to the expansion of T cells based on artificial antigen-presenting cells, which can increase the amount and quality of amplification.

Description

technical field [0001] The invention relates to the field of biological materials, in particular to a phospholipid microbead used as an artificial antigen-presenting cell, a preparation method thereof and an application thereof. Background technique [0002] In a normal immune response, antigen-specific T cells require stimulation by at least two signals to proliferate and mount an immune response to the antigen. The first signal comes from the recognition of MHC / antigen peptide complex by TCR, which transmits the antigen-specific recognition signal; the second signal is provided by costimulatory molecules of antigen-presenting cells (APC), which is a non-specific co-stimulatory signal. In the process of recognizing and binding with APC, a variety of transmembrane molecules gather on the "raft" structure rich in sphingomyelin and cholesterol, and approach each other to form clusters, forming the site where cells bind to each other. synapse. The formation of immune synapse ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01J20/26B01J20/28B01J20/30C12N5/0783
CPCB01J20/24B01J20/262B01J20/22B01J20/28021C12N5/0636C12N2501/515C12N2501/999C12N2533/76C12N2533/54C12N2533/30Y02A50/30
Inventor 杨文君黄翔
Owner CARBIOGENE THERAPEUTICS CO LTD
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