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Amplification primer of local chicken mitochondrion COI gene

A technique for amplifying primers and mitochondria, applied in the field of amplification primers

Inactive Publication Date: 2021-04-30
ANHUI AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

But so far, the COI gene of local chickens has not been widely used and has a good specific general primer

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  • Amplification primer of local chicken mitochondrion COI gene
  • Amplification primer of local chicken mitochondrion COI gene

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Embodiment Construction

[0026] The technical solutions in the embodiments of the present invention will be clearly and completely described below, obviously, the described embodiments are only some of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0027] A screening method for local chicken mitochondrial COI gene amplification primers, comprising the following steps:

[0028] (1) Log in to NCBI (http: / / www.ncbi.nlm.nih.gov / ), and download 15 existing local chicken mitochondrial COI sequences (GenBank accession numbers are: CM008858, KX987152, KP269069, KJ778617, MG605671, GU261710-GU261719);

[0029] (2) Using the sequence processing software DNAMAN 8.0 to perform multiple alignment analysis on the above sequences, and determine the conserved regions at the front and ...

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Abstract

The invention discloses an amplification primer of a local chicken mitochondrion COI gene, and belongs to the field of COI gene amplification primers, wherein the sequences of the primer comprise: COI-F: 5'-CTTTAGCCGGCAACCTAGCC-3', and COI-R: 5'-GACGTGCCTCCTTGTTAATT-3'. According to the invention, a pair of primers COI-F and COI-R for amplifying the COI sequence of the mitochondrial part of the local native chicken is screened out, a reaction system and PCR amplification conditions of the primers are determined, and experiments prove that the primers have the characteristic of good specificity, can meet the needs of research on genetic diversity of the local native chicken, and can provide reliable molecular technical guidance for germplasm resource protection, development and utilization.

Description

technical field [0001] The invention relates to the field of amplification primers, in particular to an amplification primer for local native chicken mitochondrial COI gene. Background technique [0002] Chickens are one of the most popular and widespread poultry species, providing a large amount of animal protein (meat and eggs) to humans around the world. In addition, chickens also play an important role in sociocultural and biomedical research. Native chickens are a valuable genetic resource, and their high adaptability to the local environment enables them to survive well. [0003] Endemic chickens have great genetic diversity due to worldwide selection and domestication. Genetic diversity within a species can improve adaptation to harsh environments and resistance to disease. There is no doubt that reducing the genetic diversity of native chickens is a loss of valuable genetic resources. Local native chicken populations can also add to the existing genetic diversity...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12Q1/6811C12N15/11
CPCC12Q1/6888C12Q1/6811
Inventor 金四华臧贺邱桂如贾羽晴税斐郑书丽江洪峰耿照玉
Owner ANHUI AGRICULTURAL UNIVERSITY
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