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Bacillus tequilensis, microcapsule microbial agent and preparation method

A bacillus and microcapsule technology, applied in the field of agricultural microbial inoculants, can solve the problems of damage to the bacterial cells and their natural metabolites, the reduction of the number of viable cells of biological preparations, and the reduction of the activity of bacterial agents, so as to avoid the problem of bacterial release, The effect of promoting germination and evenly dispersing bacteria

Pending Publication Date: 2021-04-20
SHENZHEN BATIAN ECOTYPIC ENG +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology provides for improved antimicrobial compositions made up of different materials called Bacteroides fragilis (C.) or Microcystis aeruginosa (Mcc). These specialized organisms help fight against harmful substances like mold spores, yeast cells, viruses, etc., while also promoting their ability to grow on plants' root systems. They form tiny structures containing various chemicals called shells around them, making it difficult for these organisms to damage any part they may be able to attack without causing illnesses if needed. By embedding this composition into another material called Braillama, we aim to create stronger protective films over both layers.

Problems solved by technology

Technically speaking, current methods for producing biofilm containing bacteamics (BAC) involve expensive processes like incubating BAC cells on solid surfaces at specific temperatures before applying them onto crops. These techniques result in reduced numbers of active ingredients within these compositions due to exposure during handling and storing. Additionally, reducing the amount of freeze dried material may affect germination rates even if no significant amounts were found.

Method used

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  • Bacillus tequilensis, microcapsule microbial agent and preparation method
  • Bacillus tequilensis, microcapsule microbial agent and preparation method
  • Bacillus tequilensis, microcapsule microbial agent and preparation method

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preparation example Construction

[0060] In a sixth aspect, the present invention provides a method for preparing a microcapsule bacterial agent, comprising the following steps:

[0061] The suspension of Bacillus tequila was mixed with sodium alginate solution, then added dropwise to CaCl 2 The solution is solidified, and then added to the chitosan solution to cover the film to prepare the microcapsule bacterial agent.

[0062] In the present invention, the Tequila bacillus is firstly dissolved in water to obtain a bacterial suspension, and then mixed with the sodium alginate solution, which contributes to the uniform dispersion of the Tequila bacillus.

[0063] The preparation method of the microcapsule bacterial agent provided by the present invention uses the sodium alginate-calcium chloride-chitosan coating system to embed Bacillus tequila, which can significantly reduce the process of preparing the microbial cell into the bacterial agent. The loss, increase the embedding rate and the stability of the ba...

Embodiment 1

[0082] Isolation and purification of microbial single colony in the soil of embodiment 1

[0083] The microorganisms in the diseased soil were separated and purified by dilution coating plate method, single cell picking method and plate streaking method. Firstly, 10 g of the collected diseased soil was weighed and added to a sterile Erlenmeyer flask filled with 90 mL of sterile water. , then place the Erlenmeyer flask on a shaker, shake it at 150rpm for 30min, let it stand for 2h, until the liquid is separated, take the supernatant for the next test, and dilute the supernatant by 10 -1 -10 -6 Gradient, 100uL of each gradient was drawn and spread on solid LB medium (recipe: tryptone 10g / L, yeast extract 5g / L, NaCl 10g / L, agar 15g / L), cultured at 28°C for 48h. After various single colonies grow on the plate, use a sterile toothpick to carefully pick a single colony, transfer it to a new LB solid medium for streaking, and then culture at 28°C for 48 hours, observe the colony sha...

Embodiment 2

[0084] The screening of embodiment 2 growth-promoting microbial strains

[0085] The screened strains were cultured in liquid, and after 24 hours of culture, the bacterial liquids of the 8 strains were diluted 1000 times, and the seed germination test was carried out. The tested seeds were selected from pakchoi seeds, and the pakchoi seeds were placed in a disposable plate. , the plate was covered with sterile gauze, according to the experimental design, 10mL of bacterial dilution was added to different plates, and then placed in a light incubator at 25°C. After 48 hours of incubation, the germination rate and germination length of the seeds were calculated. The result shows that the Tequila bacillus of the present application has the best growth-promoting ability after being diluted 1000 times, as image 3 As shown, the results of the seed germination test show that all strains have a certain growth-promoting ability compared with the control group, and the germination rates ...

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Abstract

The invention provides a bacillus tequilensis, a microcapsule microbial agent and a preparation method, and relates to the field of agricultural microbial agents. The bacillus tequilensis provided by the invention is derived from field soil, has an obvious growth promoting effect on plants, and can promote the germination of plant seeds and the growth of plant roots and stems. According to the microcapsule microbial agent provided by the invention, bacillus tequilensis is embedded through a sodium alginate-calcium chloride-chitosan coating system, so that the loss in the process of preparing microbial cells into the microbial agent can be remarkably reduced, the embedding rate and the stability of the microbial agent are improved, the activity of microorganisms in a membrane can be preserved to the maximum extent, and the growth-promoting effect of the bacillus tequilensis can be better exerted after being applied to the soil.

Description

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Claims

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Application Information

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Owner SHENZHEN BATIAN ECOTYPIC ENG
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