Total saponins of Yingbupu and its application in the preparation of drugs for treating restenosis after vascular injury

A vascular injury, eagle not fluttering technology, used in drug combinations, cardiovascular system diseases, pharmaceutical formulations, etc.

Active Publication Date: 2022-02-11
GUANGXI INT ZHUANG MEDICINE HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the prior art, there is no report about the cardiovascular protective effect of Yingbupu

Method used

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  • Total saponins of Yingbupu and its application in the preparation of drugs for treating restenosis after vascular injury
  • Total saponins of Yingbupu and its application in the preparation of drugs for treating restenosis after vascular injury
  • Total saponins of Yingbupu and its application in the preparation of drugs for treating restenosis after vascular injury

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0027]The invention provides a kind of total saponins of Yingbupu, which is prepared by the following steps: mixing the dry root bark powder of Yingbupu with an ethanol aqueous solution with a volume concentration of 70% to 80%, refluxing extraction, collecting the extract and concentrating to obtain an extract, The extract was redissolved in water and extracted with petroleum ether, the water phase was collected, and the fat-soluble components were removed, then mixed with water-saturated n-butanol for extraction, the supernatant was taken to remove n-butanol, and evaporated to dryness to obtain the total saponins of hawkbuba.

[0028] The invention uses the dried root bark of Yingbupu as an extraction material to extract the total saponins of Yingbupu. Compared with other parts of medicinal materials of Yingbupu, the dry root bark of Yingbupu has obvious advantages in the total extraction rate of the total saponins of Yingbupu. The particle size of the dried root bark powder ...

Embodiment 1

[0043] Take the dried root bark of Yingbupu, crush it into a coarse powder, weigh 200g precisely, add 2000ml 80% ethanol, reflux extraction, concentrate under reduced pressure to obtain the extract, add 500ml double distilled water to fully dissolve, transfer to a separatory funnel, and then Add 500ml of petroleum ether for extraction, take the lower aqueous phase solution in the separatory funnel, calculate the volume of the aqueous phase, add an equal volume of chloroform, shake well and let stand, take the upper layer solution in the separatory funnel, add an equal volume n-butanol, shake slightly, take the upper layer solution, repeat n-butanol extraction twice, combine the extracts, recover the solvent by rotary evaporation, and evaporate to dryness in a water bath to obtain 14 g of dry extract. It is calculated that the extraction yield of the extracted total saponins of yingbuba is 7%. Store in a brown glass bottle.

[0044] The total saponins of Yingbupu were detected...

Embodiment 2

[0046] Extraction methods and comparison of different extraction solvents for total saponins of Yingbupu

[0047] Get 2 parts of Yingbupu root bark coarse powder, each 200g, a part is reflux extracted with 70% ethanol aqueous solution, extracts according to the method for embodiment 1, another part is extracted with water (10 times of water, extract 2 times, each time 1h, the remaining steps are the same as in Example 1), and finally weigh and detect the total saponins extracted.

[0048] 12.0 g of total saponins were obtained in the alcohol extraction scheme, and the extraction rate was 6%; 3.0 g of total saponins were obtained in the water extraction scheme, and the extraction rate was 1.5%.

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Abstract

The invention provides total saponins of yippu and its application in preparing medicines for treating restenosis after vascular injury, and belongs to the technical field of vascular medicines. In the present invention, the coarse powder of dried root bark of Yingbupu is used as the material for extraction with 70% to 80% ethanol aqueous solution, and the extract is successively extracted with petroleum ether, chloroform or dichloromethane, and water-saturated n-butanol, and the The clear liquid was removed the solvent and evaporated to dryness to obtain the total saponins of Yingbupu. Based on the characteristics of total saponins of Yingbupu can effectively inhibit vascular intimal hyperplasia, inhibit the proliferation and / or transfer of vascular smooth muscle cells, and inhibit the expression of protein molecules in the Wnt signaling pathway, the present invention provides total saponins of Yingbupu in the preparation of Use of drugs in the treatment of restenosis after vascular injury.

Description

technical field [0001] The invention belongs to the technical field of vascular drugs, and in particular relates to total saponins of yingbupu and its application in the preparation of drugs for treating restenosis after blood vessel injury. Background technique [0002] Since vascular injury mostly occurs in the lumen, the research on vascular restenosis after injury almost all focuses on the intima and media. Although a lot of research results have been obtained, the exact mechanism is still unclear. Most scholars have reached a consensus: After vascular injury, the proliferation and migration of intima and media are important factors for restenosis after vascular injury. [0003] At present, there are many types of treatment for restenosis after vascular injury. For example, the patent publication No. CN104174010A discloses that knockout of SHPS1 gene can significantly promote intimal neogenesis and cell proliferation; transforming growth factor β1 plays a role in the for...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/25A61P9/10A61P9/14A61K125/00
CPCA61P9/14A61P9/10A61K36/25A61P9/08A61K2236/00
Inventor 赵湘培滕红丽钟传梅陈少锋钟文
Owner GUANGXI INT ZHUANG MEDICINE HOSPITAL
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