Gene fixed-point knock-in vector construction method, system and platform

Abstract

The invention belongs to the field of biological information, and particularly relates to a gene fixed-point knock-in vector construction method, system and platform. The method comprises the steps ofacquiring original data information of a gene to be knocked in, creating framework corresponding to the gene to be knocked in, and constructing a vector knock-in model; and acquiring sequence data tobe inserted in real time, knocking homologous arm sequences, exogenous genes and some functional elements of the gene to be knocked in into a vector in order at fixed points according to design rules. The invention further relates to a system and a platform corresponding to the method, so that fixed-point knock-in of the exogenous genes can be realized, the genetic background is simpler, the experimental operation is more accurate and efficient, and compared with traditional operation, the method is simpler, more flexible in design, lower in cost and shorter in period. In addition, the possibility is provided for the future targeted gene therapy through the fixed-point knock-in of the gene, the DNA fragment with the missing function is repaired into the DNA fragment with the function, andthe purpose of gene therapy can be achieved. Meanwhile, the output can be greatly improved, the manpower is liberated, and the working efficiency is improved.

Description

technical field [0001] The invention belongs to the field of biological information, and in particular relates to a method, system and platform for constructing a gene-directed knock-in carrier. Background technique [0002] At present, there are two main means of studying gene function: one is the loss of gene function; the other is to achieve gene overexpression. Gene knock-in is an effective technique to achieve stable gene overexpression. Gene knock-in (Gene knock-in) refers to the gene editing technology that uses random integration, transposon system, homologous recombination, etc. to insert foreign functional genes into the genome, so that they can be expressed in cells. [0003] However, the current gene knock-in models include: conventional gene knock-in, point mutation, conditional point mutation, and humanization. Earlier gene knock-in methods were mainly integrated into the host genome through random integration or transposon systems, This knock-in method is hi...

AI Technical Summary

Problems solved by technology

[0006] In view of the above-mentioned technical problems and defects that the traditional experimental operation cannot realize the fixed-point knock-in of exogenous genes, and the operation is complex and cumbersome, inefficient, time-consuming and laborious

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