A gene-directed knock-in vector construction method, system and platform

Abstract

The invention belongs to the field of biological information, and in particular relates to a method, system and platform for constructing a gene-directed knock-in carrier. The present invention obtains the original data information of the gene to be knocked in, and creates a skeleton corresponding to the gene to be knocked in, constructs a vector knock-in model; acquires the sequence data to be inserted in real time, and fixes the homology arm sequence of the gene to be knocked in, The exogenous gene and some functional elements are knocked into the carrier in an orderly manner according to the design rules, and the system and platform corresponding to the method can realize the targeted knock-in of the exogenous gene, making the genetic background simpler and the experimental operation more accurate and efficient. And it is simpler than traditional operation, more flexible in design, lower in cost, and shorter in cycle. In addition, the fixed-point knock-in of genes provides the possibility for future targeted gene therapy. Repairing functionally deficient DNA fragments into functional DNA fragments can achieve the purpose of gene therapy. At the same time, it can greatly increase output, liberate manpower, and improve work efficiency.

Description

technical field [0001] The invention belongs to the field of biological information, and in particular relates to a method, system and platform for constructing a gene-directed knock-in carrier. Background technique [0002] At present, there are two main means of studying gene function: one is the loss of gene function; the other is to achieve gene overexpression. Gene knock-in is an effective technique to achieve stable gene overexpression. Gene knock-in (Gene knock-in) refers to the gene editing technology that uses random integration, transposon system, homologous recombination, etc. to insert foreign functional genes into the genome, so that they can be expressed in cells. [0003] However, the current gene knock-in models include: conventional gene knock-in, point mutation, conditional point mutation, and humanization. Earlier gene knock-in methods were mainly integrated into the host genome through random integration or transposon systems, This knock-in method is hi...

AI Technical Summary

Problems solved by technology

[0006] In view of the above-mentioned technical problems and defects that the traditional experimental operation cannot realize the fixed-point knock-in of exogenous genes, and the operation is complex and cumbersome, inefficient, time-consuming and laborious

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