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A kind of fungal strain and application thereof for controlling alfalfa spotted aphid

A strain, alfalfa technology, applied in the direction of application, fungi, and microbial-based methods, etc., can solve problems such as unreasonable use, and achieve the effects of fast growth, large spore production, and simple cultivation

Active Publication Date: 2021-11-05
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the long-term large-scale and unreasonable use has led to different degrees of drug resistance of the alfalfa

Method used

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  • A kind of fungal strain and application thereof for controlling alfalfa spotted aphid
  • A kind of fungal strain and application thereof for controlling alfalfa spotted aphid
  • A kind of fungal strain and application thereof for controlling alfalfa spotted aphid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Example 1 Isolation of bacterial strains

[0015] The strain was derived from aphid bots collected from the fields in the western suburbs of Haidian District, Beijing. The larvae were transferred to a sterile Petri dish with wet filter paper and kept at 30°C for constant temperature cultivation. After the mycelium grows, pick the apex mycelium with a sterile inoculation loop, inoculate it on a flat plate (PDA medium), purify and cultivate it at a constant temperature of 30° C. to obtain Beauveria bassiana IPPHBB094 of the present invention, and repeat the culture for 2 Store in the laboratory at 4°C after -3 times.

[0016] 1. Tested strains: IPPHBB094, IPPBSX-1, IPPB34-3, IPPB18514, IPPB2010-15, IPPB2010-16, IPPB2010-17, IPPBFrM2019-137, IPPM189, IPPM202 were provided by the Institute of Plant Protection, Chinese Academy of Agricultural Sciences, using PDA The culture medium was separated and purified and stored in the laboratory at 4°C.

[0017] 2. Method: The germ...

Embodiment 2

[0023] Example 2 Toxicity determination of alfalfa spotted aphid population

[0024] 1. Method: inoculate by dipping method, and carry out indoor toxicity test. Use PDA to cultivate each strain at 30°C. After the spore powder covers the entire petri dish, scrape the spore powder with a spore scraper, transfer it into sterile water containing 0.01% Tween-80 to prepare a spore suspension, and place it in a shaker Shake fully on the instrument, measure the spore concentration with a hemocytometer, and dilute to the required concentration (1x10 8 spores / mL).

[0025] 2. Test strains: IPPHBB094, IPPBSX-1, IPPB34-3, IPPB18514, IPPB2010-15, IPPB2010-16, IPPB2010-17, IPPBFrM2019-137, IPPM189, IPPM202 are provided by the Institute of Plant Protection, Chinese Academy of Agricultural Sciences, using PDA The culture medium was separated and purified and stored in the laboratory at 4°C.

[0026] 3. Selection of alfalfa spot aphid for testing: alfalfa spot aphid was collected from Rishe...

Embodiment 3

[0038] The virulence strategy of embodiment 3 different bacterial strain concentrations to the spotted aphid alfalfa population

[0039] 1. Method: inoculate by dipping method, and carry out indoor toxicity test. Use PDA to cultivate each strain at 30°C. After the spore powder covers the entire petri dish, scrape the spore powder with a spore scraper, transfer it into sterile water containing 0.01% Tween-80 to prepare a spore suspension, and place it in a shaker Shake fully on the instrument, measure the spore concentration with a hemocytometer, and dilute to the required concentration (1x10 8 spores / mL).

[0040] 2. Test strains: IPPHBB094, IPPBSX-1, IPPB34-3, IPPM189, IPPM202 were all provided by the Institute of Plant Protection, Chinese Academy of Agricultural Sciences, separated and purified by PDA medium, and stored in the laboratory at 4°C.

[0041] 3. Selection of alfalfa spot aphid for testing: alfalfa spot aphid was collected from Risheng Modern Agriculture Alfalfa...

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Abstract

The invention relates to the field of plant protection, in particular to a fungal strain for preventing and treating spotted aphid alfalfa and application thereof. The preservation number of the strain is CGMCC No.19034. The bacterial strain of the present invention has the best control effect on spotted aphid of alfalfa. The culture of this strain is relatively simple, the growth speed is fast, the sporulation is large, and the spore germination rate is high. It is pathogenic to Aphid alfalfa at different times, and the pathogenic concentration is low under different concentrations. It is environmentally friendly and is not easy to produce drug resistance. It can be used for the control of spotted alfalfa aphid.

Description

technical field [0001] The invention relates to the field of plant protection, in particular to a fungal strain for preventing and treating spotted aphid alfalfa and application thereof. Background technique [0002] Aphids are one of the polyphagous pests widely distributed all over the world. They harm many crops and economic plants. The main one that harms alfalfa is Aphid alfalfa. Dwarfing, slow growth, when severe, the young shoots wither, destroy chlorophyll, excrete honeydew to reduce photosynthesis and cause germs to breed, and can also spread viral diseases. Due to its short growth cycle and large reproductive capacity, it will explode rapidly when the conditions are suitable, causing immeasurable losses to agriculture and animal husbandry. [0003] At present, the production mainly relies on chemical agents to prevent and control the aphid alfalfa, and the existing indoor insecticidal activity tests are to screen some chemical agents with higher effects. However,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/30A01P7/04A01G13/00C12R1/645
CPCA01G13/00A01N63/30
Inventor 王广君陈佳杰田野张泽华农向群李红梅涂雄兵李贝贝李霜
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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