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Biological feed containing functional oligosaccharide and preparation method thereof

A biological feed and functional technology, applied in the field of livestock and poultry feed, can solve the problems of complex process and low fructooligosaccharide content

Pending Publication Date: 2021-03-19
博益德(北京)生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This type of method is relatively complicated in technology, and it needs to go through pretreatment, decolorization, concentration and other processes when obtaining functional oligosaccharides, and the content of fructooligosaccharides produced is low

Method used

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  • Biological feed containing functional oligosaccharide and preparation method thereof
  • Biological feed containing functional oligosaccharide and preparation method thereof
  • Biological feed containing functional oligosaccharide and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0101] Embodiment 1, preparation contains the biological feed of functional oligosaccharide

[0102] Refer to attached figure 1 , to prepare biological feed containing functional oligosaccharides.

[0103] 1 Preparation of functional oligosaccharides

[0104] 1) Dilute molasses: take molasses in a fermenter, dilute with water at a mass ratio of 1:1, adjust the pH to 5.5 with sodium hydroxide to obtain a molasses solution;

[0105] 2) According to 0.8U / g molasses solution, add β-fructosyltransferase, and carry out enzymatic hydrolysis reaction at 55°C and pH 5.5;

[0106] 3) At 6 hours of enzymolysis, according to 8U / g molasses solution, glucose oxidase was added to continue enzymolysis, the total time of enzymolysis reaction was 18h, and an enzymolysis solution containing fructooligosaccharides (molasses solution after enzymolysis) was obtained.

[0107] Fructose-oligosaccharides, also known as fructo-oligosaccharides, are kestose, kestose and kestrose formed by combining 1...

Embodiment 2

[0136] Embodiment 2. Feeding test

[0137] Feeding test:

[0138] Test site: a pig farm in Tianjin

[0139] In the experiment, 16 24-day-old "Du×Long×Da" weaned piglets with an initial body weight of (7.15±0.02) kg were randomly assigned to the control group (n=8) and the test group (n=8). Piglets in the control group were fed basal diets that met the nutritional requirements of piglets specified in the NRC feeding standards. Piglets in the test group were fed the basal diet supplemented with 8% (mass content) of the biofermented feed containing functional oligosaccharides prepared in Example 1. The test period is 14 days.

[0140] Table 3 Effect of bio-fermented feed containing functional oligosaccharides on growth performance of piglets

[0141]

[0142] Note: No letter on the shoulder of peer data indicates no significant difference (P>0.05), and different letters indicate significant difference (P<0.05). The table below is the same.

[0143] It can be seen from Ta...

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Abstract

The invention discloses a biological feed containing functional oligosaccharide and a preparation method of the biological feed. The method comprises the following steps of 1, putting molasses into afermentation tank, diluting with water, and regulating the pH value to 5-6; adding beta-fructosyl transferase into the molasses solution, and carrying out an enzymolysis reaction; when enzymolysis iscarried out for 1-8 hours, adding glucose oxidase for asynchronous enzymolysis and obtaining functional oligosaccharide; and 2, mixing the raw materials, wherein the raw material mixture is prepared from the following substances of, in percentage by mass, 5 to 15 percent of enzymolyzed molasses, 10 to 30 percent of corn, 5 to 15 percent of bran, 20 to 30 percent of soybean meal, 15 to 25 percent of peanut meal and 12 to 25 percent of hybrid paper mulberry, adding a complex microbial inoculant and a complex enzyme preparation into the mixture for fermentation to obtain a fermentation product, namely the biological feed containing the functional oligosaccharide. When the obtained biological feed is applied to weaned pigs, the growth performance of the weaned pigs can be improved, and the intestinal redox capacity and small intestine mucosa are improved.

Description

technical field [0001] The invention belongs to the technical field of livestock and poultry feed, and specifically relates to a method for preparing biological feed containing functional oligosaccharides by using molasses as a raw material, performing asynchronous solid-state fermentation with bacteria and enzymes after enzymatic hydrolysis. Background technique [0002] Fructose-oligosaccharide is a natural active substance with a sweetness of 0.3 to 0.6 times that of sucrose. It is an activation and proliferation factor for bifidobacteria in the intestinal tract of animals. It can significantly improve the proportion of microbial populations in the intestinal tract, reduce and inhibit the growth of harmful bacteria, regulate Intestinal flora balance, etc. [0003] Sucrose molasses is a by-product of sugar cane sugar production, accounting for about 3% of the total sugar cane. It contains about 30% sucrose. Using molasses to prepare fructo-oligosaccharides has the advant...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/12A23K10/14A23K10/30A23K10/37A23K20/22A23K20/24A23K50/30A23K50/60
CPCA23K10/14A23K10/12A23K10/30A23K10/37A23K20/22A23K20/24A23K50/30A23K50/60Y02P60/87
Inventor 张妮妮陈达程善燕刘世杰任婉丽杨继帅黄爽
Owner 博益德(北京)生物科技有限公司
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