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Lipoprotein (a) detection kit

A technology for detecting kits and lipoproteins, which is applied in biological testing, measuring devices, and material inspection products. Conducive to early diagnosis and prognostic treatment, improve the effect of accuracy and stability

Inactive Publication Date: 2021-03-12
广州市奥宇科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, it may lead to large differences in test results and low accuracy, which is not suitable for widespread promotion and application.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1, a kind of lipoprotein (a) detection kit

[0034] The preparation of the lipoprotein (a) detection kit

[0035] 1. The preparation method of the antibody microtiter plate is as follows:

[0036] Dilute the goat anti-human apolipoprotein (a) polyclonal antibody to 10 μg / ml with a 0.05 mol / L Tris-HCl buffer solution with a pH of 8.0, and add it to a 96-well microtiter plate in an amount of 100 μl / well. Place it overnight at a temperature of 4°C, pour off the coating solution, add a blocking solution for blocking, the blocking solution is composed of a mixture of 0.2% BSA and 3g / L ethylphenyl polyethylene glycol, dry in vacuum, and seal , that is.

[0037] 2. The preparation method of the biotin-antibody-coated plate is as follows:

[0038] Mix goat anti-human apolipoprotein (a) polyclonal antibody with activated biotin for labeling, and dialyze to remove unbound biotin to obtain biotin goat anti-human apolipoprotein (a) polyclonal antibody; pH ​​9.5 Dilut...

Embodiment 2

[0046] Embodiment 2, a kind of lipoprotein (a) detection kit

[0047] The preparation of the lipoprotein (a) detection kit

[0048] 1. The preparation method of the antibody microtiter plate is as follows:

[0049] Dilute the goat anti-human apolipoprotein (a) polyclonal antibody to 10 μg / ml with a 0.05 mol / L Tris-HCl buffer solution with a pH of 8.0, and add it to a 96-well microtiter plate in an amount of 100 μl / well. Place it overnight at a temperature of 4°C, pour off the coating solution, add a blocking solution for blocking, the blocking solution is composed of a mixture of 0.2% BSA and 4g / L ethylphenyl polyethylene glycol, dry in vacuum, and seal , that is.

[0050] 2. The preparation method of the biotin-antibody-coated plate is as follows:

[0051] Mix goat anti-human apolipoprotein (a) polyclonal antibody with activated biotin for labeling, and dialyze to remove unbound biotin to obtain biotin goat anti-human apolipoprotein (a) polyclonal antibody; pH ​​9.5 Dilut...

Embodiment 3

[0059] Embodiment 3, a kind of lipoprotein (a) detection kit

[0060] The preparation of the lipoprotein (a) detection kit

[0061] 1. The preparation method of the antibody microtiter plate is as follows:

[0062] Dilute the goat anti-human apolipoprotein (a) polyclonal antibody to 10 μg / ml with a 0.05 mol / L Tris-HCl buffer solution with a pH of 8.0, and add it to a 96-well microtiter plate in an amount of 100 μl / well. Place it overnight at a temperature of 4°C, pour off the coating solution, add a blocking solution for blocking, the blocking solution is composed of a mixture of 0.2% BSA and 5g / L ethylphenyl polyethylene glycol, dry in vacuum, and seal , that is.

[0063] 2. The preparation method of the biotin-antibody-coated plate is as follows:

[0064] Mix goat anti-human apolipoprotein (a) polyclonal antibody with activated biotin for labeling, and dialyze to remove unbound biotin to obtain biotin goat anti-human apolipoprotein (a) polyclonal antibody; pH ​​9.5 Dilut...

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Abstract

The invention belongs to the technical field of biological detection, and particularly relates to a lipoprotein (a) detection kit. The lipoprotein (a) detection kit provided by the invention is mainlycomposed of an antibody elisa plate, a biotin antibody coated plate, horse radish peroxidase-streptavidin, lipoprotein (a) series standard substances, a diluent, a cleaning solution, a substrate solution, a developing solution and a stop solution. The lipoprotein (a) detection kit provided by the invention has the advantages of simple operation, high sensitivity and strong anti-interference capability, can effectively reduce the interference of other substances in serum, especially the interference effect of an anti-RF factor on a detection result, and greatly improves the accuracy and stability of the detection result. Meanwhile, the kit can also reduce the phenomenon of inaccurate detection results caused by the difference of the copy number of apolipoprotein (a) molecules KringleIV-2 in lipoprotein (a), and is more beneficial to early diagnosis and prognosis treatment of patients with arteriosclerosis and heart diseases.

Description

technical field [0001] The invention belongs to the technical field of biological detection, in particular to a lipoprotein (a) detection kit. Background technique [0002] Lipoprotein (a) [Lipoprotein (a), Lp (a)] is a unique lipoprotein in the human body, with low-density lipoprotein (LDL)-like particles and apolipoprotein (a) [Apolipoprotein (a), apo (a)] composition. At present, a large number of epidemiological and clinical studies have proved that the level of Lp(a) has a continuous independent moderate degree of correlation with heart disease and ischemic stroke, although Lp(a) exists in the blood of different populations. However, its concentration in the serum of the same individual is relatively stable, and it is hardly affected by age, sex, smoking, diet, lipid metabolism, drugs, environment and other factors except genetic factors. Therefore, high Lp(a) can be used as an independent risk factor for cardiovascular and cerebrovascular atherosclerotic diseases. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/92G01N33/543G01N33/535G01N33/532G01N33/53
CPCG01N33/5306G01N33/532G01N33/535G01N33/54306G01N33/92G01N2333/47G01N2800/324
Inventor 黄秀珍
Owner 广州市奥宇科技有限公司
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