Lipoprotein (a) detection kit
A technology for detecting kits and lipoproteins, which is applied in biological testing, measuring devices, and material inspection products. Conducive to early diagnosis and prognostic treatment, improve the effect of accuracy and stability
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Embodiment 1
[0033] Embodiment 1, a kind of lipoprotein (a) detection kit
[0034] The preparation of the lipoprotein (a) detection kit
[0035] 1. The preparation method of the antibody microtiter plate is as follows:
[0036] Dilute the goat anti-human apolipoprotein (a) polyclonal antibody to 10 μg / ml with a 0.05 mol / L Tris-HCl buffer solution with a pH of 8.0, and add it to a 96-well microtiter plate in an amount of 100 μl / well. Place it overnight at a temperature of 4°C, pour off the coating solution, add a blocking solution for blocking, the blocking solution is composed of a mixture of 0.2% BSA and 3g / L ethylphenyl polyethylene glycol, dry in vacuum, and seal , that is.
[0037] 2. The preparation method of the biotin-antibody-coated plate is as follows:
[0038] Mix goat anti-human apolipoprotein (a) polyclonal antibody with activated biotin for labeling, and dialyze to remove unbound biotin to obtain biotin goat anti-human apolipoprotein (a) polyclonal antibody; pH 9.5 Dilut...
Embodiment 2
[0046] Embodiment 2, a kind of lipoprotein (a) detection kit
[0047] The preparation of the lipoprotein (a) detection kit
[0048] 1. The preparation method of the antibody microtiter plate is as follows:
[0049] Dilute the goat anti-human apolipoprotein (a) polyclonal antibody to 10 μg / ml with a 0.05 mol / L Tris-HCl buffer solution with a pH of 8.0, and add it to a 96-well microtiter plate in an amount of 100 μl / well. Place it overnight at a temperature of 4°C, pour off the coating solution, add a blocking solution for blocking, the blocking solution is composed of a mixture of 0.2% BSA and 4g / L ethylphenyl polyethylene glycol, dry in vacuum, and seal , that is.
[0050] 2. The preparation method of the biotin-antibody-coated plate is as follows:
[0051] Mix goat anti-human apolipoprotein (a) polyclonal antibody with activated biotin for labeling, and dialyze to remove unbound biotin to obtain biotin goat anti-human apolipoprotein (a) polyclonal antibody; pH 9.5 Dilut...
Embodiment 3
[0059] Embodiment 3, a kind of lipoprotein (a) detection kit
[0060] The preparation of the lipoprotein (a) detection kit
[0061] 1. The preparation method of the antibody microtiter plate is as follows:
[0062] Dilute the goat anti-human apolipoprotein (a) polyclonal antibody to 10 μg / ml with a 0.05 mol / L Tris-HCl buffer solution with a pH of 8.0, and add it to a 96-well microtiter plate in an amount of 100 μl / well. Place it overnight at a temperature of 4°C, pour off the coating solution, add a blocking solution for blocking, the blocking solution is composed of a mixture of 0.2% BSA and 5g / L ethylphenyl polyethylene glycol, dry in vacuum, and seal , that is.
[0063] 2. The preparation method of the biotin-antibody-coated plate is as follows:
[0064] Mix goat anti-human apolipoprotein (a) polyclonal antibody with activated biotin for labeling, and dialyze to remove unbound biotin to obtain biotin goat anti-human apolipoprotein (a) polyclonal antibody; pH 9.5 Dilut...
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