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Multi-diagnostic kit

A diagnostic kit, the technology of the kit, which is applied in the direction of DNA/RNA fragment, microbial determination/inspection, recombinant DNA technology, etc., can solve the problems of lack of rapid detection method and non-detection, and achieve reasonable design of primer length and Tm value. , the mismatch rate is low, and the effect of improving the amplification efficiency

Inactive Publication Date: 2021-02-23
新开源汇诚(武汉)医疗科技有限公司
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  • Abstract
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  • Claims
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Problems solved by technology

CYP1B1 has multiple polymorphic sites, and site mutations are of great significance for the screening of metabolic diseases, but currently there is no clinical detection of this index, and there is no effective rapid detection method

Method used

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Embodiment Construction

[0029] The CYP1B1 multi-diagnosis kit provided by the present invention will be further described in detail and completely below in conjunction with the examples. The embodiments described below are exemplary only for explaining the present invention and should not be construed as limiting the present invention.

[0030] The experimental methods in the following examples are conventional methods unless otherwise specified. The experimental materials used in the following examples were purchased from the market unless otherwise specified.

[0031] 1. Design primers

[0032] Primers were designed according to the polymorphic sites published in NCBI, and the rs10012 site was a "C / G" base mutation, represented by "S". The specific sequence is as follows:

[0033] CCTCTTCAGA TGGATTATTA CAGGTAGCGG GTGGCGTGGT AGGTACTTTA AAGGAAATCAAGCGCCACCG CCTCGATGCC CGCAGCGTTG TCCCCAGATT GCAGGAACCG TTACGCGCCT TGCGGGGAGGGGAAGGGTTT GGCGCTGGGT TACAGCGAGG TGGAAACACG CCCCTTCTCT TCTCCAAGGG AGAGTGGGTTG...

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Abstract

The invention discloses a multi-diagnostic kit. The kit comprises five pairs of amplification primers and a multi-PCR mixed reaction solution, wherein the amplification primers are used for amplifyingCYP1B1 polymorphic sites, including an rs10012 site, an rs1056836 site, an rs1056827 site and / or an rs1056836 site; and each pair of primers corresponds to upstream and downstream areas of an SNP site. According to the multi-diagnostic kit, a multi-PCR amplification method is adopted, five important SNP sites of CYP1B1 are amplified at the same time, and amplified site combinations can be flexibly selected according to actual needs, so that the kit has multiple functions; the primers in the kit are high in specificity and low in mismatching rate, the primer length and Tm value are reasonablydesigned, and amplified products are convenient to detect and separate; and a plurality of target products are amplified at a time, so that the amplification efficiency is greatly improved, and the amplification cost is reduced. The detection kit can be expanded and extended to other SNP site detection and has good popularization values.

Description

technical field [0001] The invention relates to a multi-diagnosis kit, which belongs to the field of clinical detection kits. Background technique [0002] As a new member of the CYP450 family, CYP1B1 can be expressed in liver and extrahepatic tissues. CYP1B1 not only participates in the metabolism of many exogenous substances such as polycyclic aromatic hydrocarbons, but also participates in the metabolism of many endogenous metabolites in the body, including the metabolism of steroid hormones, fatty acids, melatonin and vitamins. In addition, CYP1B1 interacts with many nuclear receptors such as PPARs, RAR, and ER to jointly maintain the metabolic homeostasis of important physiological substances in the body. In recent years, many research results have shown that the occurrence and development of obesity, hypertension, atherosclerosis and cancer can be significantly prevented by regulating CYP1B1. Clearly, CYP1B1 has become an important target for the treatment of metabol...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883C12Q1/6886C12Q1/6858C12N15/11
CPCC12Q1/6858C12Q1/6883C12Q1/6886C12Q2600/156C12Q2600/16C12Q2531/113C12Q2537/143
Inventor 张辉
Owner 新开源汇诚(武汉)医疗科技有限公司
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