A sliding microfluidic organ chip and its preparation method

Abstract

The invention provides a sliding microfluidic organ chip and a preparation method thereof, comprising: a sliding assembly and a microfluidic chip base, the sliding assembly is provided with a sliding rod, a glue injection port, a sealing plug and an embedded tissue chamber, and the injection The glue port is located directly above the embedded tissue chamber and communicates with the embedded tissue chamber, and the extracellular matrix is ​​injected into the top of the glue injection port, and the extracellular matrix is ​​filled into the embedded tissue chamber; the microfluidic chip base has a Sliding groove, first and second exchange channels, first and second liquid reservoirs, push the sliding rod into the bottom of the sliding groove, at this time, the two sides of the embedded tissue chamber are facing the first and second exchange channels respectively , the first fluid reservoir, the first exchange channel, the embedded tissue chamber, the second exchange channel, and the second fluid reservoir communicate with each other. The invention can realize stable and reliable perfusion of the extracellular matrix without leakage; can realize the barrier-free contact between the extracellular matrix and the cell culture medium, and is beneficial to realize uniform and stable fluid characteristics in the extracellular matrix.

Description

technical field [0001] The invention belongs to a microfluidic chip in the technical field of biomedical engineering, and in particular relates to a sliding microfluidic organ chip and a preparation method thereof. Background technique [0002] Compared with traditional two-dimensional cell culture models and animal models with species differences, the human organ chip integrates human cells and hydrogels into the microfluidic chip to further simulate the construction of multiple controllable microenvironments in the body on the chip. Finally, in vitro realization of specific human organ microtissues with three-dimensional microstructure and microphysiological functions has attracted much attention. Therefore, this technology has been widely used in the fields of disease modeling, drug screening and personalized medicine. [0003] Usually, the three-dimensional culture model based on the perfusion mode is widely used in the construction of human-like organ chips. Its basic s...

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Problems solved by technology

[0007] In summary, the currently reported microfluidic organ chips have certain limitations in terms of reliable extracellular matrix perfusion, uniform distribution of fluid in tissue chambers, and cell / microtissue extraction. A new type of organ chip is urgently needed. Human organ chip design to enhance the stability and flexibility of related operations

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