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Lipophilic microbial nutritional agent

A nutrient and lipophilic technology, applied in the field of lipophilic microbial nutrient, can solve the problems affecting microbial growth, petroleum hydrocarbon utilization ability and loss, and achieve the effect of increasing bacterial growth, improving alkane degradation rate, and high sample loading

Active Publication Date: 2021-02-05
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] After oil spill pollution occurs, a large amount of input of carbon sources will change the carbon-nitrogen ratio in the environment. Microorganisms will use these excess carbon as growth substrates and rapidly lose available inorganic nutrients such as N and P, thereby affecting the growth of microorganisms. and the ability to utilize petroleum hydrocarbons

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0042] A preparation method of lipophilic microbial nutrient,

[0043] Add sodium alginate and additives to the ethanol solution, stir at 25°C for 20 minutes to obtain a wall material solution, add nutrients to the wall material solution, stir and mix thoroughly for 30 minutes to obtain a nutrient-wall material mixed solution, and mix the nutrients- The wall material mixed solution is added to the paraffin liquid containing the active agent, fully stirred and mixed for 20 minutes, added to the calcium chloride solution for fixation, fully stirred and mixed for 40 minutes, centrifuged at 25°C, washed with acetone, dried and solidified to obtain lipophilic microbial nutrition agent; the mass fraction of ethanol solution is 30wt%, and the addition amount of sodium alginate is 2wt% of ethanol solution, and additive comprises nancandle xyloside and rhamnoside, and the addition amount of nancandle xyloside is sodium alginate 15wt%, the rhamnosyl ester is 2-O-rhamnopyranosyl-rhamnopy...

Embodiment 2

[0045] A preparation method of lipophilic microbial nutrient,

[0046]Add sodium alginate and additives to the ethanol solution, stir at 25°C for 20 minutes to obtain a wall material solution, add nutrients to the wall material solution, stir and mix thoroughly for 30 minutes to obtain a nutrient-wall material mixed solution, and mix the nutrients- The wall material mixed solution is added to the paraffin liquid containing the active agent, fully stirred and mixed for 20 minutes, added to the calcium chloride solution for fixation, fully stirred and mixed for 40 minutes, centrifuged at 25°C, washed with acetone, dried and solidified to obtain lipophilic microbial nutrition agent; the mass fraction of ethanol solution is 30wt%, and the addition amount of sodium alginate is 2wt% of ethanol solution, and additive comprises nancandle xyloside and rhamnoside, and the addition amount of nancandle xyloside is sodium alginate 25wt%, the rhamnosyl ester is 2-O-rhamnopyranosyl-rhamnopyr...

Embodiment 3

[0048] A preparation method of lipophilic microbial nutrient,

[0049] Add sodium alginate and additives to the ethanol solution, stir at 25°C for 20 minutes to obtain a wall material solution, add nutrients to the wall material solution, stir and mix thoroughly for 30 minutes to obtain a nutrient-wall material mixed solution, and mix the nutrients- The wall material mixed solution is added to the paraffin liquid containing the active agent, fully stirred and mixed for 20 minutes, added to the calcium chloride solution for fixation, fully stirred and mixed for 40 minutes, centrifuged at 25°C, washed with acetone, dried and solidified to obtain lipophilic microbial nutrition agent; the mass fraction of ethanol solution is 30wt%, and the addition amount of sodium alginate is 2wt% of ethanol solution, and additive comprises nancandle xyloside and rhamnoside, and the addition amount of nancandle xyloside is sodium alginate 35wt%, the rhamnosyl ester is 2-O-rhamnopyranosyl-rhamnopy...

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Abstract

The invention discloses a lipophilic microbial nutritional agent, and belongs to the technical field of slow-release nutritional agents. The prepared nutritional agent comprises a slow-release carrierwith cross-linked sodium alginate as a wall material, an additive dispersed in the slow-release carrier, and nutritional components embedded in the slow-release carrier, wherein the additive containslyoniside. As the lyoniside and rhamnolipid are adopted in preparation of the nutritional agent, the obtained nutritional agent is high in total nitrogen and total phosphorus sample loading capacity,the total nitrogen sample loading capacity is 9.7% or above, and the total phosphorus sample loading capacity is 6.6% or above; and the slow release effect is good, the slow release time can reach 24h or above, and the total phosphorus dissolution rate is 70-82% after 24 h of release.

Description

technical field [0001] The invention belongs to the technical field of slow-release nutrient, and in particular relates to a lipophilic microbial nutrient. Background technique [0002] With the rapid growth of demand for oil in various countries around the world, the amount of offshore oil extraction and the offshore oil transportation industry have increased rapidly. Although science and technology are constantly improving, oil spills often occur in the process of oil extraction, refining, transportation and storage. ACCIDENT. Petroleum hydrocarbon pollution has gradually become another major pollutant in the world's oceans (especially shallow seas) besides nutrients. Petroleum is mainly composed of alkanes, aromatic hydrocarbons, and cycloalkanes, in addition to compounds containing oxygen, sulfur, and nitrogen, and non-hydrocarbon compounds such as colloids and asphaltenes. When an oil spill occurs at sea, the oil will be photodegraded to produce toxic oxides such as q...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/38C12N1/20
CPCC12N1/38C12N1/20
Inventor 竺柏康孙午阳张乐李翠翠陶亨聪黄江华董钦炳
Owner ZHEJIANG OCEAN UNIV
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