Tissue culture propagation method of cinnamomum japonicum
A technique of Tianzhu cinnamon and tissue culture, which can be used in horticultural methods, botanical equipment and methods, horticulture, etc., and can solve problems such as few reports.
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[0063] A tissue culture propagation method of Tianzhu Gui, which comprises the steps of:
[0064] 1) Selection of female parent material: 50 annual seedlings were selected as the female parent of micropropagation explants;
[0065] The method of cultivating annual seedlings is to collect seeds in the previous year before October 15th, sow them as they are harvested, and use the sand storage method. After the seeds germinate, they are transplanted into containers for cultivation.
[0066] 2) Cultivation environment: Greenhouse cultivation is adopted, the light intensity range is 2000-3000lx, and the temperature range is 12-36°C.
[0067] 3) Moisture control: The leaf surface humidity is controlled at 20%-50%, and the soil humidity is controlled at 40%-90%.
[0068] 4) Cultivation method: use container cultivation, the container is 50cm away from the ground, and 1 / 4 Hoagland's nutrient solution is applied to keep the plant's diameter section tender green, and the axillary buds ...
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