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Exosome sorting method for NK cell activation stage

A technology of NK cells and exosomes, applied in the biological field, can solve the problem of directional sorting of umbilical cord blood specific exosomes without technology, and achieve the effect of preserving integrity, good biological safety and good repeatability

Pending Publication Date: 2021-01-22
曹峰林
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0011] The purpose of the present invention is to solve the problem that there is no technology for directional sorting of umbilical cord blood-specific exosomes, and to provide a method for sorting exosomes in the activation stage of NK cells

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  • Exosome sorting method for NK cell activation stage
  • Exosome sorting method for NK cell activation stage
  • Exosome sorting method for NK cell activation stage

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Embodiment Construction

[0054] Those of ordinary skill in the art can understand that the above-mentioned embodiments are specific examples for realizing the present invention, and in practical applications, various changes can be made to it in form and details without departing from the spirit and spirit of the present invention. scope.

[0055] In order to make the purpose, technical solutions and advantages of the embodiments of the present invention clearer, the spirit of the disclosure of the present invention will be described in detail below. After any person skilled in the art understands the embodiments of the present invention, they can learn from the present invention The techniques taught by the summary of the invention are subject to change and modification without departing from the spirit and scope of the summary of the invention.

[0056] The exemplary embodiments and descriptions of the present invention are used to explain the present invention, but not to limit the present inventio...

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Abstract

The invention discloses an exosome sorting method for an NK cell activation stage, and relates to the field of biology. The invention aims to solve the problem that no technology for directionally sorting specific exosomes of umbilical cord blood exists at present. According to the invention, cell exosome separation in the NK cell period ofthe umbilical cord blood after specific stimulation and activation is adopted, NK cells are proliferated through IL-2, IL-12, IL-15, GM-SCF and Fit3-L can promote activation of the NK cells, and CD34<+> stem cells can be induced to differentiate into the NKcells; the NK cells are sorted and collected on about the 10th day of in-vitro culture so as to obtain more exosomes secreted by the NK cells; the characteristics of an innovative optimized NK cell factor culture method and feeding method are utilized, and the activated NK cell exosomes are extracted on a large scale and used for achieving the purpose of activating the NK cells; and the method isapplied to the field of exosomes.

Description

technical field [0001] The invention relates to the field of biology, in particular to a method for sorting exosomes in the activation stage of NK cells. Background technique [0002] The pathogenesis of viral infection or tumor is mainly due to the inability of the body's immune ability to defeat pathogenic microorganisms or remove tumor cells. Human natural killer cells (NK cells) are the body's own natural killer immune cells. They are the first line of defense against viral infections and clearing aging diseased cells. It has a clear role in the occurrence process, and its main immunological feature is the production of cytokines, mediating cytotoxic activity through the secretion of cytokines such as IFN-γ, and mediating the killing of target cells through the release of perforin and granzymes, (Vivieret al., 2008). NK cells are the core cells in the innate immune system, accounting for about 15% of white blood cells in the blood, are crucial to both innate and adapti...

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Application Information

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IPC IPC(8): C12N5/0783C12N5/0775
CPCC12N5/0646C12N5/0665C12N2509/00C12N2500/90C12N2501/2302C12N2501/2312C12N2501/2315C12N2501/125C12N2501/998C12N2500/38C12N2501/26C12N2502/1164C12N2502/137Y02A50/30
Inventor 曹韪凡滕春波于泽曹峰林陈京京曹涵
Owner 曹峰林
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