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Application of rice gene OsDES1

A rice and gene technology, applied in the field of genetic engineering and plant genetic breeding, can solve problems such as examples of unsuccessful applications

Active Publication Date: 2021-01-08
CHINA NAT RICE RES INST
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the intelligent restoration system is still only a conception, and no successful application examples have appeared.

Method used

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  • Application of rice gene OsDES1
  • Application of rice gene OsDES1
  • Application of rice gene OsDES1

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Example 1 Function of Rice Fertility Gene OsDES1

[0062] 1. Phenotype analysis of des1 mutants

[0063] A low seed-setting rate mutant des1 was identified and isolated from the mutant library obtained by radiation mutagenesis in Zhonghui 8015. The mutant has a stable trait with reduced seed setting rate, and the mutant has a decreased seed setting rate in Fuyang, Zhejiang and Lingshui, Hainan. During the vegetative growth period, compared with the wild type, the mutant had no significant difference in plant type and tillering, and the plant height was slightly reduced ( figure 1 a). During the reproductive growth period, compared with the wild type, the heading stage of the mutant was earlier than that of the wild type, and part of the neck ( figure 2 a), the ear is slightly brown, and the ear length becomes shorter ( figure 1 b). There was no significant difference in appearance and morphology of mutant pistils compared with wild type ( figure 1 c). Compared w...

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Abstract

The invention discloses an application of a rice gene OsDES1. The invention discloses for the first time that the rice gene OsDES1 has a biological function of regulating and controlling plant fertility. The function of the gene is verified in rice based on a gene knockout experiment and an overexpression experiment, a CRISPR / Cas9 gene knockout vector is constructed to transform Nipponbare of rice, female gametes of transgenic plants grow abnormally, and fertility is reduced; after the pCUBi1390-OSDES1 overexpression vector is used for converting a mutant des1, the fertility of the female gamete of the transgenic plant is recovered, namely, the female plant is partially aborted due to OSDES1 gene function loss type mutation, and the fertility is recovered to be normal through the overexpression of the gene. The rice OSDES1 gene is not completely aborted after the function is lost, part of seeds can be harvested, the rice OSDES1 gene can form favorable supplementation with other femalesterile genes, and a new gene resource is provided for rice intelligent restorer line breeding.

Description

technical field [0001] The invention relates to the field of genetic engineering and plant genetic breeding, in particular to the application of the rice gene OsDES1. Background technique [0002] As the most important food crop, rice provides nearly 50% of human food and nutrition sources. More than half of the population in my country takes rice as their main food. The popularization of hybrid rice has made a huge contribution to the food security of the world. In the 1970s, the discovery of the landmark wild male sterility gene promoted the promotion of three-line hybrid rice breeding, and a large number of hybrid rice with strong heterosis were bred. hybrid combination. The utilization of heterosis is divided into "three-line method" hybrid rice represented by nuclear-cytoplasmic interaction pollen sterile and "two-line method" hybrid rice represented by photothermo-sensitive genic male sterile. However, the "three-line method" is restricted by strict restoration and ...

Claims

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Application Information

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IPC IPC(8): C12N15/29C12N15/82C12N15/113C07K14/415A01H5/00A01H6/46
CPCC07K14/415C12N15/113C12N15/8289C12N15/8218C12N15/8261
Inventor 张迎信程式华胡霞曹立勇于萍孙廉平
Owner CHINA NAT RICE RES INST
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