Establishment of cytoskeleton binding protein WDR1 gene knockout model and application of model

A technology that binds protein and cytoskeleton, applied in the field of transgenic

Inactive Publication Date: 2012-05-30
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] So far, the function of WDR1 in lower organisms has been widely revealed. However, some questions remain about the role of this important active protein in higher mammals and whether it is related to certain diseases. not resolved

Method used

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  • Establishment of cytoskeleton binding protein WDR1 gene knockout model and application of model
  • Establishment of cytoskeleton binding protein WDR1 gene knockout model and application of model
  • Establishment of cytoskeleton binding protein WDR1 gene knockout model and application of model

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Establishment of knockout model of cytoskeleton-binding protein gene

[0054] 1. By constructing a gene targeting vector, see figure 1 ;

[0055] 2. Transfect embryonic liver cells (ES) with the above-mentioned vectors, and screen homologously recombined ES cell clones by Southern blot hybridization, see figure 2 ;

[0056] 3. Injecting the homologously recombined ES cells obtained in step (2) into blastocysts, implanting them into the uterus of pseudopregnant mice, and obtaining chimeric mice after the offspring are born;

[0057] 4. The chimeric mice were further mated to obtain the knockout mice of cytoskeleton binding protein WDR1.

Embodiment 2

[0059] Phenotypic Analysis of Cytoskeleton Binding Protein Knockout Mice

[0060] The WDR1 gene-deficient mice obtained in Example 1 had no obvious abnormalities at birth, and all died on the 14th day after birth; compared with wild-type control mice, the heart weight / body weight ratio of WDR1 gene knockout mice increased significantly ; Histological section staining analysis results showed that the heart of WDR1 gene knockout mice was significantly larger than that of wild-type control mice, see image 3

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Abstract

The invention discloses a preparation method of a cytoskeleton binding protein WDR1 gene knockout non-human mammal model and application of the model. The invention also discloses a method for screening compounds for preventing and treating cardiomegaly by utilizing the cytoskeleton binding protein WDR1 gene knockout non-human mammal model and application of the WDR1 and upstream and downstream biological signal transduction accesses as medicine intervention target spots to treatment of cardiomegaly.

Description

technical field [0001] The invention relates to the field of transgenic technology, and more specifically relates to the establishment and application of a cytoskeleton binding protein WDR1 gene knockout animal model. Background technique [0002] The protein encoded by the WDR1 gene is a WD repeat protein, which belongs to the WD40 repeat domain protein family. The WD40 repeat sequence is a structural motif consisting of about 30-40 amino acids, containing several conserved amino acid residues, mainly including tryptophan and aspartic acid residues (W-D) at the carbon termini. WD repeat protein is a class of proteins containing 4-16 WD repeat sequences, and it is a protein family that exists in all eukaryotes. The basic function of the members of this family is to act as a scaffold for the connection between proteins and play a coordinating role in the assembly of multiprotein complexes. Studies have shown that WDR1 protein can bind to the shredded protein / actin complex; ...

Claims

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Application Information

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IPC IPC(8): C12N15/85A01K67/027A61K49/00A61K38/17A61P9/00
Inventor 万萍张力军袁白银陈炯
Owner NANJING UNIV
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