Preparation and application of bacillus amyloliquefaciens capable of preventing and treating tobacco bacterial wilt

A technology for dissolving starch spores and tobacco bacterial wilt disease, applied in application, tobacco cultivation, bacteria and other directions, can solve problems such as disease, drug resistance, affecting personal safety, etc., to shorten fermentation time, reduce environmental pollution, and avoid drug resistance. The effect of mutation

Pending Publication Date: 2021-01-05
JIANGXI SHUNQUAN BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, bacterial wilt caused by bacteria such as Laueria is the most serious soil-borne disease on tobacco. Tobacco bacterial wilt caused by bacteria cannot be effectively inhibit

Method used

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  • Preparation and application of bacillus amyloliquefaciens capable of preventing and treating tobacco bacterial wilt
  • Preparation and application of bacillus amyloliquefaciens capable of preventing and treating tobacco bacterial wilt
  • Preparation and application of bacillus amyloliquefaciens capable of preventing and treating tobacco bacterial wilt

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Example Embodiment

[0025]Example 1

[0026]A Bacillus amyloliquefaciens capable of preventing and controlling bacterial wilt of tobacco, characterized in that the beneficial microorganism used is Bacillus amyloliquefaciens, which has been deposited in the General Microbiology Center of the Chinese Microbial Strain Collection Management Committee.

[0027]Classification: Bacillus amyloliquefaciens; Bacillus;

[0028]Deposit number: CGMCC No.6462;

[0029]Preservation address: No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing;

[0030]Date of preservation: August 21, 2012.

[0031]The preparation of Bacillus amyloliquefaciens capable of preventing and curing tobacco bacterial wilt is characterized in that it comprises the following steps:

[0032]① The strain is activated, and the cryopreserved Bacillus amyloliquefaciens is inoculated into a plate medium in a sterile environment. The composition of the plate medium and its mass percentage: peptone 5.0%, yeast extract 3.0%, sodium chloride 1.0%, The agar powder is ...

Example Embodiment

[0038]Example 2

[0039]Application of Bacillus amyloliquefaciens capable of preventing and curing tobacco bacterial wilt

[0040]Test site and name: The test site is located at the tobacco test site in Shangluo City, Shaanxi Province; a kind of Bacillus amyloliquefaciens inocula for the control of tobacco bacterial wilt;

[0041]Test materials: ①500-fold dilution of Bacillus amyloliquefaciens of the present invention; ②500-fold dilution of Bacillus subtilis inoculum; ③commercially available chemical pesticides; ④clear water CK control;

[0042]Test treatment: The field test was chosen to be carried out in the tobacco test site in Shangluo City because of the fact that tobacco has been planted in this site over the years, the incidence of bacterial wilt has been serious, the soil fertility is medium and high, and there is a good planting foundation. The test variety is Yunyan. The above test materials were sprayed on the base of the tobacco stem before transplanting. Each tobacco plant used 100...

Example Embodiment

[0057]Example 3

[0058]Application of Bacillus amyloliquefaciens capable of preventing and curing tobacco bacterial wilt

[0059]Test site and name: The test site is located at the tobacco test site in Shangluo City, Shaanxi Province; a kind of Bacillus amyloliquefaciens inoculation against tobacco mosaic virus;

[0060]Test materials: ①500-fold dilution of Bacillus amyloliquefaciens of the present invention; ②500-fold dilution of Bacillus subtilis inoculum; ③commercially available chemical pesticides; ④clear water CK control;

[0061]Test treatment: The test variety is Yunyan. The above test materials are sprayed on the base of the tobacco stem before transplanting. Each tobacco plant uses 100ml of reagent. After transplanting, the tobacco is regularly applied with root irrigation and spraying techniques. The amount of each application is the same and the same as the previous year's application amount, normal cultivation. Set up 3 test plots for each reagent to reduce the accidental error of ...

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Abstract

The invention discloses preparation and application of bacillus amyloliquefaciens capable of preventing and treating tobacco bacterial wilt, particularly relates to preparation and application of bacillus amyloliquefaciens capable of preventing and treating tobacco bacterial wilt, and particularly relates to beneficial microbial bacteria which are bacillus amyloliquefaciens. The bacillus amyloliquefaciens is preserved in China General Microbiological Culture Collection Center with the number of CGMCC No.6462. The adopted fermentation process is a liquid fermentation process, bacillus amyloliquefaciens powder is prepared by adopting more advanced ultrafiltration concentration and spray drying modes, and preparation is conducted by adopting an ultrafiltration concentration mode, so that thelive spore count of a product can quickly reach 100 billion/g. Through a proper use method, the bacillus amyloliquefaciens is fixed on the rhizosphere, the body surface and the like of a plant, competes for surrounding nutrients with pathogenic bacteria and secretes antibacterial substances to inhibit growth and propagation of the pathogenic bacteria, and meanwhile, a plant self-regulating systemcan be enhanced to resist invasion of the pathogenic bacteria, so that the purpose of biocontrol is achieved.

Description

technical field [0001] The invention belongs to the field of agricultural microorganism prevention and control, and in particular relates to the preparation and application of a bacillus amyloliquefaciens capable of preventing and treating tobacco bacterial wilt. Background technique [0002] Tobacco bacterial wilt is one of the main diseases of tobacco in tropical and subtropical regions. It occurs commonly in the Yangtze River Basin and south of China, including Guangdong, Fujian, Taiwan, Hunan, Jiangxi, eastern Guangxi, southern Anhui, Sichuan and Guizhou. The damage caused by local smoked areas is serious, and outbreaks often occur in individual years, causing devastating losses. In recent years, the incidence and range of damage of the disease has a tendency to expand to the northern smoking areas, and it has occurred in Shandong, Henan, Shaanxi, Liaoning and other provinces, and the damage is serious in some areas. The loss caused by this disease ranks the 4th of all ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/02A01N63/22A01P1/00A01G13/00A01G22/45C12R1/07
CPCC12N1/20C12N1/02A01N63/22A01G13/00A01G22/45C12R2001/07C12N1/205
Inventor 陈文安德荣
Owner JIANGXI SHUNQUAN BIOTECH CO LTD
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