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29-plex Y-STR typing system for family search and paternal biogeographic ancestor inference

A technology of physical evidence and Y chromosome, applied in the field of forensic science, can solve the problems of unclear application strategy of Y-STR loci in forensic medicine, lack of Y-STR typing system, etc.

Pending Publication Date: 2020-12-29
ACADEMY OF FORENSIC SCIENCE
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AI Technical Summary

Problems solved by technology

3) The forensic application strategy of the rapidly mutating Y-STR locus is not yet clear
[0009] In summary, there is still a lack of a satisfactory Y-STR typing system suitable for the Chinese population

Method used

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  • 29-plex Y-STR typing system for family search and paternal biogeographic ancestor inference
  • 29-plex Y-STR typing system for family search and paternal biogeographic ancestor inference
  • 29-plex Y-STR typing system for family search and paternal biogeographic ancestor inference

Examples

Experimental program
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Effect test

Embodiment 1

[0200] Example 1: Establishment and optimization of 29 Y-STR multiplex amplification systems

[0201] For the detailed information of each locus of the 29 Y-STR multiplex amplification systems, such as the GeneBank accession number, core repeat sequence, and allele range, see Table 1 above.

[0202] Detailed primer information for each locus is given in Table 2 above.

[0203] After the primer design is completed, a single locus is amplified first, and the electrophoretic typing results determine whether the primer design is good or bad. The criteria for primer design failure are incomplete addition of A phenomenon, PCR non-specific peaks, PCR false peaks, and low signal. peak and no product peak. After the primers are determined, adjust the primer concentration, and then repeatedly explore the conditions and optimize, so that 29 loci can be multiplexed and amplified in one reaction system at the same time, and finally the electrophoretic typing results shall prevail.

[020...

Embodiment 2

[0215] Example 2: Forensic Evaluation of 29 Y-STR Multiplex Amplification Systems

[0216] 2.1. System sensitivity

[0217] Male standard 9948 DNA was diluted into 8 different concentration gradients: 2ng, 1ng, 500pg, 250pg, 125pg, 62.5pg, 1.25pg and 15.625pg. Under the same reaction conditions, the detection of the 29Y-STR multiplex amplification system was carried out respectively. The results show that the multiplex amplification system for 29 Y-STR loci can obtain the complete typing results of 29 Y-STR loci when the template amount is higher than 0.125ng, and the loci are lost when the template amount is less than 0.125ng . When the template amounts were 62.5pg, 31.25pg and 15.625pg, the average detection rates of locus alleles were 95%, 78.16% and 47.13%, see Figure 7 . When the template amount was 62.5pg, the average allele loss rate was 5%. With the decrease of the template amount, the allele loss rate increased from 21.84% (31.25pg) to 52.87% (15.625pg ).

[02...

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Abstract

The invention provides a 29-plex Y-STR typing system for family search and paternal biogeographic ancestor inference. Specifically, a Y chromosome short tandem repeat typing kit provided by the invention contains detection reagents for specifically detecting Y chromosome STR loci in the following groups (a), (b) and (c) that: the group (a) includes DYS19, DYS389I, DYS389II, DYS390, DYS391, DYS392,DYS393, DYS385, DYS437, DYS438, DYS439, DYS448, DYS456, DYS458, DYS635 and Y-GATA-H4; the group (b) includes DYS526a, DYS526b, DYS570, DYS576 and DYS626; and the group (c) includes DYS388, DYS460, DYS481, DYS593, DYS596, DYS643 and DYS645. The typing system disclosed by the invention has the advantages of simplicity in operation, easiness in typing reading, high amplification specificity, balanced typing result peak value, reliable and stable performance and the like.

Description

technical field [0001] The invention belongs to the technical field of forensic medicine, and in particular relates to a 29-plex Y-STR typing system for family search and paternal biogeographic ancestry inference. Background technique [0002] Short tandem repeats (short tandem repeats, STR), also known as microsatellite DNA or simple sequence repeats (SSR), are currently the most widely used length polymorphic genetic markers in forensic evidence identification. The unit (also known as the core sequence) is short, only 2 to 6 bp, and consists of the head-to-tail tandem repeated arrangement of the core sequence. STR-PCR typing technology based on PCR technology has become the most mainstream means of contemporary individual identification and paternity testing. [0003] Forensic DNA typing technology provides an important means for case detection. The theory and method based on short tandem repeat sequence STR genetic markers have become the mainstream technology of forens...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888
CPCC12Q1/6888C12Q2600/156
Inventor 边英男黄磊李成涛张素华王新杰李金林韩清顺李敏
Owner ACADEMY OF FORENSIC SCIENCE
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