Composition for preventing or treating nicotine addiction, which contains liriope latifolia extract as active ingredient

A technology of Ophiopogon japonicus extract and M. broadleaf, which is applied in the field of drugs for preventing or treating nicotine addiction, can solve the problem that there is no literature report of Ophiopogon japonicus, and there is no composition for preventing or treating symptoms of nicotine addiction. Healthy functional food And other issues

Active Publication Date: 2020-12-08
KOREA RES INST OF CHEM TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, so far, no literature has reported the effect of Liriope platyphylla on improving the symptoms of nicotine addiction
In addition, so far, there have been no patents on compositions for the prevention or treatment of nicotine addiction symptoms and health functional foods related to Ophiopogon japonicus

Method used

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  • Composition for preventing or treating nicotine addiction, which contains liriope latifolia extract as active ingredient
  • Composition for preventing or treating nicotine addiction, which contains liriope latifolia extract as active ingredient
  • Composition for preventing or treating nicotine addiction, which contains liriope latifolia extract as active ingredient

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0095] Embodiment 1: Preparation of the hot water extract of Ophiopogon japonicus

[0096] The root of Ophiopogon japonicus was dried, and 2.1 L of water was added to 300 g of dried Ophiopogon japonicus root, which was then extracted twice in a reflux device at 80° C. for 4 hours. After reflux and filtration, the extract was freeze-dried under reduced pressure. The results showed that 197g of the hot water extract of Ophiopogon japonicus was obtained, and the yield was 65%.

Embodiment 2

[0097] Embodiment 2: the preparation of the ethanol extract of Ophiopogon japonicus broadleaf

[0098] Preparation of 30% ethanol extract of Ophiopogon japonicus

[0099] The root of Ophiopogon japonicus was dried, and 2.1 L of 30% ethanol (v / v) was added to 300 g of dried Ophiopogon japonicus root, and it was extracted twice in a reflux device at 70° C. for 4 hours. After reflux and filtration, the extract was freeze-dried under reduced pressure. The results showed that 191g of the 30% ethanol extract of Ophiopogon japonicus was obtained, and the yield was 63%.

[0100] Preparation of 50% ethanol extract of Ophiopogon japonicus

[0101] Dry the root of Radix Ophiopogon japonicus, and add 2.1 L of 50% ethanol (v / v) to 300 g of the dried root of Ophiopogon japonicus, and extract it twice in a reflux device at 60° C. for 4 hours . After reflux and filtration, the extract was freeze-dried under reduced pressure. The results showed that 171 g of the 50% ethanol extrac...

experiment example 1

[0102] Experimental Example 1: Measuring the concentration of neuronal cell line (PC-12 cells) cell activity in

[0103] The cell viability of the neuronal cell line (PC-12 cell line) according to the treatment concentrations of nicotine and Ophiopogon japonicus extract was determined by MTT assay.

[0104] Specifically, PC-12 cells were cultured in DMEM medium (Dulbecco's modified Eagle's medium) supplemented with 10% fetal bovine serum (FBS), 5% horse serum and 1% antibiotics (Thermo fisher company, Cat. No. 15140122) at 37 °C, 5% CO 2 cultured in an incubator. Distribute the cultured PC-12 cells in 1 x 10 4 Cells / well density in 96-well plates and further cultured in the same medium and conditions for 24 hr. Then, PC-12 cells were treated with the nicotine (1, 10, 100 and 1000 μM / ml) and the extract of Ophiopogon japonicus (1, 10, 100 and 1000 μg / ml) of Example 1 for 24 hours, respectively. Then, 20 μl of the dye solution (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxyme...

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PUM

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Abstract

The invention relates to an application of a liriope latifolia extract in preparation of a medicine for preventing or treating nicotine addiction. The present invention is accomplished by confirming that a liriope latifolia extract can be effectively used as an active ingredient for preventing and treating nicotine addiction, and the liriope latifolia extract regulates changes in the expression level of tyrosine hydroxylase (TH) protein and dopamine transporter (DAT) protein caused by nicotine, and the increase of spontaneous activity of nicotine addicted rats is inhibited.

Description

technical field [0001] The present invention relates to the application of the extract of Liriope platyphylla in the preparation of medicine for preventing or treating nicotine addiction. Background technique [0002] According to a report by the Ministry of Health and Welfare, 22.6% of Korean adults (men: 39.3%, women: 5.5%) are long-term smokers. Exposure to harmful substances from smoking, which today is considered the leading cause of cancer, shortens average life expectancy by about 8 to 10 years. According to statistics, one-third of people who die from cancer are smokers. In particular, it is known that 90% of male lung cancer patients are caused by smoking in Korea. In addition, smoking is believed to be directly or indirectly involved in the development of various cancers such as bladder, pancreas, throat, cervix, and esophagus. [0003] Despite the negative effects of smoking, the reason for continuing to smoke is the addictive nature of nicotine (Philos TransR ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/8968A61P25/34A61P25/20A61P25/22A61P25/24A23L33/105
CPCA61K36/8968A61P25/34A61P25/20A61P25/22A61P25/24A23L33/105A23V2002/00A23V2200/322A23V2200/332A61K36/896A61P25/30
Inventor 徐廷旭贾敏汉柳仁秀金泰完林日娜李大永李映燮金錦淑尹多慧潘在淑
Owner KOREA RES INST OF CHEM TECH
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