Method for increasing yield of beta-carotene produced by fermentation of Blakeslea trispora

Abstract

The invention belongs to the technical field of microbial fermentation, and particularly belongs to a method for increasing the yield of beta-carotene produced by fermentation of Blakeslea trispora. When Blakeslea trispora is used for liquid submerged fermentation, an ethephon solution with a certain concentration is added, ethephon slowly releases ethylene, and ethylene plays a role in promotingcarotenoid synthesis related genes on the transcription level, so that accumulation of a large amount of beta-carotene is promoted, and the fermentation yield of beta-carotene is increased. The methodis low in cost, simple and convenient to operate and obvious in effect, and can replace various fermentation accelerators such as an oxygen carrier, a surfactant, a precursor substance and the like to increase the yield of beta-carotene, so that other fermentation accelerators are prevented from being too high in price and poison to strains, and a good basis is provided for industrial production.Compared with a blank control group, the yield of beta-carotene is increased by 54%-78%, and meanwhile, the fermentation period is shortened to 3 days from the original 5 days and is shortened by 40%.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation, in particular to a method for increasing the yield of beta-carotene produced by fermentation of Brassella trispora. Background technique [0002] In the prior art, when B. trispora produces β-carotene by fermentation, various fermentation accelerators such as oxygen carriers, surfactants, and precursors are usually added to increase the fermentation yield of β-carotene. However, the disadvantage of adding the above-mentioned substances during the fermentation process is that the purchase cost of the above-mentioned fermentation accelerators is generally high, and at the same time, some accelerators are toxic to fermentation strains. Contents of the invention [0003] In view of the above technical problems, the purpose of the present invention is to provide a method for improving the yield of β-carotene produced by the fermentation of B. trispora, using B. trispora to carry out l...

AI Technical Summary

Problems solved by technology

[0002] In the prior art, when B. trispora produces β-carotene by fermentation, various fermentation accelerators such as oxygen carriers, surfactants, and precursors are usually added to increase the fermentation yield of β-carotene. However, the disadvantage of adding the above-mentioned substances in the fermentation process is that the purchase cost of the above-mentioned fermentation accelerators is generally high, and some accelerators are toxic to the fermentation bacteria.