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A bionic artificial temporomandibular joint disc and its preparation method

A temporomandibular joint and artificial technology, applied in the field of bionic artificial temporomandibular joint disc and its preparation, can solve the problems of difficult reconstruction, high cost, inability to match the physiological load of temporomandibular joint, etc., and achieves low preparation cost, low cost, clinical The effect of high conversion value

Active Publication Date: 2021-08-31
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the above-mentioned problems existing in the prior art, the present invention provides a bionic artificial temporomandibular joint disc and its preparation method, in which the bio-ink is prepared from the decellularized intervertebral disc, supplemented with polymer materials, and the temporomandibular joint is reconstructed by 3D printing method Disc, which effectively solves the problems in the prior art that cannot match the physiological load of the temporomandibular joint, difficult reconstruction and high cost

Method used

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  • A bionic artificial temporomandibular joint disc and its preparation method
  • A bionic artificial temporomandibular joint disc and its preparation method
  • A bionic artificial temporomandibular joint disc and its preparation method

Examples

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Effect test

Embodiment 1

[0030] A bionic artificial temporomandibular joint disc, the preparation method of which comprises the following steps:

[0031] (1) The intervertebral discs of 8-month-old oxtails were harvested under sterile conditions, washed with penicillin saline to remove blood stains, then frozen and thawed for 5 cycles at a temperature range of -20 to 37°C, and extracted with ultrapure water / guanidine hydrochloride chemical reagents Glycosaminoglycan and protein, and then break the remaining tissue into floc, add 4wt% sodium dodecyl sulfate (SDS) and 4KU / ml DNase to treat at room temperature for 2 hours, wash with enzyme-free water, and obtain intervertebral disc floc sample;

[0032] (2) Freeze-dry the intervertebral disc flocculent sample obtained in step (1), then add the digestive solution, blend at room temperature and 25r / min for 60h, adjust the pH value to neutral with sodium hydroxide, and then add the step (1) The resulting glycosaminoglycan and protein are used to obtain bio...

Embodiment 2

[0036] A bionic artificial temporomandibular joint disc, the preparation method of which comprises the following steps:

[0037] (1) The intervertebral discs of 8-month-old oxtails were harvested under sterile conditions, washed with penicillin saline to remove blood stains, then frozen and thawed for 5 cycles at a temperature range of -20 to 37°C, and extracted with ultrapure water / guanidine hydrochloride chemical reagents Glycosaminoglycans and proteins, and the remaining tissues were crushed into flocculents, treated with 5wt% sodium dodecyl sulfate and 5KU / ml DNase at room temperature for 5 hours, washed with enzyme-free water, and intervertebral disc flocculent samples were obtained;

[0038](2) Freeze-dry the intervertebral disc flocculent sample obtained in step (1), then add the digestive solution, blend at room temperature and 30r / min for 72h, adjust the pH value to neutral with sodium hydroxide, and then add the step (1) The resulting glycosaminoglycan and protein ar...

Embodiment 3

[0041] A bionic artificial temporomandibular joint disc, the preparation method of which comprises the following steps:

[0042] (1) The intervertebral discs of 8-month-old oxtails were harvested under sterile conditions, washed with penicillin saline to remove blood stains, then frozen and thawed for 5 cycles at a temperature range of -20 to 37°C, and extracted with ultrapure water / guanidine hydrochloride chemical reagents Glycosaminoglycan and protein, and then crush the remaining tissue into flocculent, add 6wt% sodium lauryl sulfate and 6KU / ml DNase to treat at room temperature for 4 hours, wash with enzyme-free water, and obtain flocculent intervertebral disc samples;

[0043] (2) Freeze-dry the intervertebral disc flocculent sample obtained in step (1), then add the digestive solution, blend at room temperature and 35r / min for 84 hours, adjust the pH value to neutral with sodium hydroxide, and then add the step (1) The resulting glycosaminoglycan and protein are used to ...

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Abstract

The invention provides a bionic artificial temporomandibular joint disc and a preparation method thereof. The preparation method comprises the following steps: collecting the intervertebral disc of oxtail, cleaning it, then freezing and thawing it, extracting glycosaminoglycan and protein, breaking it into flocculent form, Add sodium lauryl sulfate and DNase to treat at room temperature, wash, and obtain intervertebral disc flocculent samples; freeze-dry the intervertebral disc flocculent samples, then add digestive juice, blend, adjust the pH value to neutral, and then add glycosaminoglycans Sugar and protein are used to obtain bio-ink; polymer materials and bio-ink are blended at room temperature for 3D printing to obtain a bionic artificial temporomandibular joint disc. The present invention also includes the bionic artificial temporomandibular joint disc prepared by the method. The present invention uses decellularized intervertebral discs to prepare bio-ink, supplemented by polymer materials, and uses 3D printing to reconstruct the temporomandibular joint disc, which effectively solves the physiological load, reconstruction difficulty and high cost of the temporomandibular joint that cannot be matched in the prior art. question.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials, and in particular relates to a bionic artificial temporomandibular joint disc and a preparation method thereof. Background technique [0002] Temporomandibular joint disk (Temporomandibular joint disk, TMJ disk) is a fibrocartilage-like tissue located between the mandibular condyle and the glenoid fossa of the temporal bone. Distributes loads, cushions shocks, and reduces dissonance between condyle and glenoid bony structures. Clinically, the destruction or loss of the temporomandibular joint disc is one of the main causes of temporomandibular joint diseases, which in turn will cause abnormal function of the entire stomatognathic system and seriously affect the quality of life of patients. According to relevant data, there are as many as one million patients in the world who need to reconstruct the articular disc every year, and the medical expenses required are also as high as hund...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/36A61L27/50A61L27/22A61L27/20A61F2/30
CPCA61F2/3099A61F2002/30985A61F2002/30993A61L27/20A61L27/22A61L27/3658A61L27/3687A61L27/3691A61L27/50A61L2430/24A61L2430/40C08L89/00C08L5/00
Inventor 祝颂松姜楠毕瑞野王旻应彬彬陈浩哲
Owner SICHUAN UNIV
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