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Materials and methods for biosynthetic manufacture of pimelic acid and utilization of synthetic polypeptides

A technology of biological and biological origin, which is applied in the fields of biochemical equipment and methods, introduction of foreign genetic material and enzymes using vectors, etc.

Pending Publication Date: 2020-11-13
INVISTA TEXTILES (U K) LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no economically favorable petrochemical pathway to produce 7-AHA

Method used

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  • Materials and methods for biosynthetic manufacture of pimelic acid and utilization of synthetic polypeptides
  • Materials and methods for biosynthetic manufacture of pimelic acid and utilization of synthetic polypeptides
  • Materials and methods for biosynthetic manufacture of pimelic acid and utilization of synthetic polypeptides

Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach 1

[0187] Embodiment 1: A kind of polypeptide or functional fragment thereof with acyl-acyl carrier protein (ACP) thioesterase (TE) activity, wherein said polypeptide with acyl-ACP TE activity comprises a relative to wild-type acyl-ACP TE or multiple amino acid substitutions, wherein the one or more amino acid substitutions are located at positions 5, 32, 33, 35, 36, 38, 40, 45, 59, 64, 90, 111, 128 occupying the same position as SEQ ID NO: 1 , 175 and 241 at the amino acids corresponding to the positions.

Embodiment approach 2

[0188] Embodiment 2: The embodiment according to embodiment 1, wherein the wild-type acyl-ACP TE is an acyl-ACP TE classified as EC 3.1.2.-.

Embodiment approach 3

[0189] Embodiment 3: The embodiment according to embodiment 1 or 2, wherein the wild-type acyl-ACP TE is an acyl-ACP TE classified as EC 3.1.2.14.

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PUM

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Abstract

Provided herein are novel, synthetic polypeptides having, for example, acyl-acyl carrier protein (ACP) thioesterase (TE) activity, including polypeptides that convert pimeloyl-ACP to pimelic acid. Insome aspects, the synthetic polypeptides have advantageous enzymatic activity and / or improved substrate specificity relative to a wild type acyl-ACP TE.

Description

[0001] priority claim [0002] This application claims the benefit of U.S. Provisional Patent Application No. 62 / 650,629, filed March 30, 2018, which is hereby incorporated by reference for all purposes. technical field [0003] The teachings in this disclosure relate to novel synthetic polypeptides having, for example, acyl-acyl carrier protein (ACP) thioesterase (TE) activity, including polypeptides that convert pimeloyl-ACP to pimelic acid. In some aspects of the disclosure, the synthetic polypeptides have favorable enzymatic activity and / or improved substrate specificity relative to wild-type acyl-ACP TE. Background technique [0004] Biochemical pathways are used to produce products useful to an organism. See eg LEHNINGER, A.L., NELSON, D.L., & COX, M.M. (2000). Lehninger principles of biochemistry. New York, Worth Publishers. The biochemical pathway to produce 7-aminoheptanoic acid (7-AHA) utilizes the biotin biosynthesis pathway in Escherichia coli (E. coli) (Lin et...

Claims

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Application Information

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IPC IPC(8): C12N9/10C12N9/16C12N15/70C12N15/82
CPCC12N9/16C12N15/70C12P7/40C12P7/44C12P13/005C12Y301/02014
Inventor 亚历山大·布雷特·福斯特阿尔贾·巴曼乔纳森·肯尼迪保罗·S·珀尔曼
Owner INVISTA TEXTILES (U K) LTD
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